Dissection of a co-translational nascent chain separation event

被引：32

作者：

Doronina, Victoria A. ^{[1
]}

de Felipe, Pablo ^{[2
]}

Wu, Cheng ^{[3
]}

Sharma, Pamila ^{[1
]}

Sachs, Matthew S. ^{[3
]}

Ryan, Martin D. ^{[2
]}

Brown, Jeremy D. ^{[1
]}

机构：

[1] Univ Newcastle Upon Tyne, RNA Biol Grp, Inst Cell & Mol Biosci, Newcastle Upon Tyne NE2 4HH, Tyne & Wear, England

[2] Univ St Andrews, Sch Biol, Ctr Biomol Sci, St Andrews KY16 9ST, Fife, Scotland

[3] Texas A&M Univ, Dept Biol, College Stn, TX 77843 USA

来源：

BIOCHEMICAL SOCIETY TRANSACTIONS | 2008年 / 36卷

基金：

英国医学研究理事会; 美国国家卫生研究院; 英国生物技术与生命科学研究理事会;

关键词：

2A peptide; cis-acting hydrolase element (CHYSEL); recoding; translation; yeast;

D O I：

10.1042/BST0360712

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Some RNA and protein sequences are capable of directing changes to the course of translation from that expected from the mRNA sequence, and this process is termed translational 'recoding'. 'CHYSEL' peptides are similar to 19-amino-acid sequences found in many viral genomes. When translated at internal portions of polypeptides, they yield co-translational separation of the nascent Chain at their C-termini. We dissected the reaction promoted by CHYSEL sequences using yeast genetics and in vitro translation systems. Our results indicate that the reaction occurs within the peptidyltransferase centre of the ribosome where the nascent chain is hydrolytically released from tRNA despite the presence of further sense codons.

引用

页码：712 / 716

页数：5

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