Gastric infusion of short-chain fatty acids can improve intestinal barrier function in weaned piglets

被引：131
作者：
Diao, H. [1 ,2 ]
Jiao, A. R. [1 ]
Yu, B. [1 ]
Mao, X. B. [1 ]
Chen, D. W. [1 ]
机构：
[1] Sichuan Agr Univ, Inst Anim Nutr, Xinkang Rd 46, Yaan 625014, Sichuan, Peoples R China
[2] Sichuan Acad Anim Sci, Anim Breeding & Genet Key Lab Sichuan Prov, Chengdu, Sichuan, Peoples R China
来源：
GENES AND NUTRITION | 2019年 / 14卷 / 1期
基金：
中国国家自然科学基金; 国家高技术研究发展计划(863计划);
关键词：
Short-chain fatty acids; Intestinal growth; Apoptotic; Gut barrier; Piglets; EPITHELIAL-CELL PROLIFERATION; GROWTH-PERFORMANCE; SODIUM-BUTYRATE; DIETARY FIBER; PIGS; METABOLISM; MORPHOLOGY; EXPRESSION; HEALTH; BACTERIA;
D O I：
10.1186/s12263-019-0626-x
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
BackgroundThe present study was conducted to investigate the effects of gastric infusion of short-chain fatty acids (SCFA) on gut barrier function in a pig model. In this study, 21 DLY barrows with an average initial body weight of (8.310.72) kg were randomly allotted into three treatments: (1) control, (2) infusing low SCFA, S1, (3) infusing high SCFA, S2. The experimental period lasted for 7days.ResultsGastric infusion of SCFA increased the concentrations of SCFA in serum and digesta, and enhanced the mRNA and protein abundances of SCFA receptors in pig intestine (P<0.05). Moreover, gastric infusion of SCFA led to alteration of intestinal morphology, elevation of intestinal development-related gene abundances, and decrease of apoptotic cell percentage, as well as reduction of pro-apoptosis gene and protein abundances (P<0.05). Besides, the jejunal SLC(7)A(1) and ileal DMT1 mRNA abundances in the SCFA infusion groups were higher than those in the control group (P<0.05). Additionally, gastric infusion of SCFA increased the mRNA abundances of Occludin and Claudin-1 in the duodenum and ileum, enhanced Lactobacillus spp counts in the ileal digesta, decreased the mRNA and protein abundances of IL-1 in the colon, and reduced Escherichia coli count in the ileal digesta (P<0.05).Conclusions These data indicated that gastric infusion of SCFA, especially high SCFA concentration, may be beneficial to gut development of piglets via improving gut morphology, decreasing apoptotic cell percentage, and maintaining intestinal barrier function.
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页数：16
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