CD8+ T Cells Mediate the Athero-Protective Effect of Immunization with an ApoB-100 Peptide

被引：82

作者：

Chyu, Kuang-Yuh ^{[1
]}

Zhao, Xiaoning ^{[1
]}

Dimayuga, Paul C. ^{[1
]}

Zhou, Jianchang ^{[1
]}

Li, Xiaojun ^{[1
]}

Yano, Juliana ^{[1
]}

Lio, Wai Man ^{[1
]}

Chan, Lai Fan ^{[1
]}

Kirzner, Jonathan ^{[1
]}

Trinidad, Portia ^{[1
]}

Cercek, Bojan ^{[1
]}

Shah, Prediman K. ^{[1
]}

机构：

[1] Cedars Sinai Med Ctr, Div Cardiol, Oppenheimer Atherosclerosis Res Ctr, Cedars Sinai Heart Inst, Los Angeles, CA 90048 USA

来源：

PLOS ONE | 2012年 / 7卷 / 02期

关键词：

ATHEROSCLEROTIC LESIONS; APOLIPOPROTEIN B-100; DENDRITIC CELLS; DEFICIENT MICE; ADOPTIVE TRANSFER; IMMUNE-RESPONSES; LDL IMMUNIZATION; DENSITY; SEQUENCES; POPULATIONS;

D O I：

10.1371/journal.pone.0030780

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Immunization of hypercholesterolemic mice with selected apoB-100 peptide antigens reduces atherosclerosis but the precise immune mediators of athero-protection remain unclear. In this study we show that immunization of apoE (-/-) mice with p210, a 20 amino acid apoB-100 related peptide, reduced aortic atherosclerosis compared with PBS or adjuvant/carrier controls. Immunization with p210 activated CD8(+) T cells, reduced dendritic cells (DC) at the site of immunization and within the plaque with an associated reduction in plaque macrophage immunoreactivity. Adoptive transfer of CD8(+) T cells from p210 immunized mice recapitulated the athero-protective effect of p210 immunization in naive, non-immunized mice. CD8(+) T cells from p210 immunized mice developed a preferentially higher cytolytic response against p210-loaded dendritic cells in vitro. Although p210 immunization profoundly modulated DCs and cellular immune responses, it did not alter the efficacy of subsequent T cell dependent or independent immune response to other irrelevant antigens. Our data define, for the first time, a role for CD8(+) T cells in mediating the athero-protective effects of apoB-100 related peptide immunization in apoE (-/-) mice.

引用

页数：12

共 40 条

[1] Natural regulatory T cells control the development of atherosclerosis in mice [J].