Zone shape and concentration in isotachophoresis-capillary electrophoresis

被引：0
|
作者：
Chen, SJ [1 ]
Graves, SW [1 ]
Lee, ML [1 ]
机构：
[1] Brigham Young Univ, Dept Chem & Biochem, Provo, UT 84602 USA
来源：
JOURNAL OF MICROCOLUMN SEPARATIONS | 1999年 / 11卷 / 05期
关键词：
isotachophoresis; capillary electrophoresis; zone shape;
D O I：
10.1002/(SICI)1520-667X(1999)11:5<341::AID-MCS3>3.0.CO;2-#
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The relationship between injection volume of a dilute sample and its concentration at peak maximum in an isotachophoresis (ITP) zone is described. The concentration at peak maximum is predicted to increase linearly with injection volume for a given leading electrolyte concentration as long as the analyte mass injected is very small. In addition, the relationship between sensitivity of counterflow isotachophoresis-capillary zone electrophoresis and injection volume was investigated using angiotensins as test analytes. The results confirm that sensitivity is a nearly linear function of injection volume for dilute samples. (C) 1999 John Wiley & Sons, Inc.
引用
收藏
页码：341 / 345
页数：5
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