Hemolytic Effect of Polymeric Nanoparticles: Role of Albumin

被引：41

作者：

Barshtein, G. ^{[1
]}

Arbell, D. ^{[2
]}

Yedgar, S. ^{[1
]}

机构：

[1] Hebrew Univ Jerusalem, Fac Med, Dept Biochem, IL-91120 Jerusalem, Israel

[2] Hadassah Hebrew Univ Hosp, Dept Pediat Surg, IL-91120 Jerusalem, Israel

来源：

IEEE TRANSACTIONS ON NANOBIOSCIENCE | 2011年 / 10卷 / 04期

关键词：

Erythrocyte; hemolysis; nanoparticles; CELL-FREE HEMOGLOBIN; PROTEIN ADSORPTION; LATEX-PARTICLES; SIZE; ELECTROPHORESIS; PREECLAMPSIA; CHARGE;

D O I：

10.1109/TNB.2011.2175745

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Nanoparticles (NP) have drawn increasing interest from many fields in medicine and are a relatively new class of biomedical products. Because there are concerns about the health effects of nanoparticles, it is important to understand how nanoparticles interact specifically with red blood cells (RBC), a central object in the blood circulation. As numerous studies that have examined NP/RBC interaction concentrated on the hemolytic potential of nanoparticles, we describe an investigation of hemolytic activity of polystyrene nanoparticles (PS-NP) in protein free medium and its modulation by albumin. We found that treatment of RBCs with PS-NP induces hemolysis (dose and particle size dependent) in plasma free medium but not in full plasma or in buffer, which contain albumin. Critical albumin concentration is 0.05% wt. According to our results hemolytic effect of nanoparticles is strongly modulated by protein concentration in the medium.

引用

页码：259 / 261

页数：3

共 23 条

[1] COLLOIDAL CARRIERS FOR INTRAVENOUS DRUG TARGETING - PLASMA-PROTEIN ADSORPTION PATTERNS ON SURFACE-MODIFIED LATEX-PARTICLES EVALUATED BY 2-DIMENSIONAL POLYACRYLAMIDE-GEL ELECTROPHORESIS [J].

BLUNK, T ;

HOCHSTRASSER, DF ;

SANCHEZ, JC ;

MULLER, BW ;

MULLER, RH .

ELECTROPHORESIS, 1993, 14 (12) :1382-1387

[2] Detailed identification of plasma proteins adsorbed on copolymer nanoparticles [J].

Cedervall, Tommy ;

Lynch, Iseult ;

Foy, Martina ;

Berggard, Tord ;

Donnelly, Seamas C. ;

Cagney, Gerard ;

Linse, Sara ;

Dawson, Kenneth A. .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2007, 46 (30) :5754-5756

[3]

Chambers E, 2007, EXP BIOL MED, V232, P958

[4] The influence of size and charge of chitosan/polyglutamic acid hollow spheres on cellular internalization, viability and blood compatibility [J].

Dash, Biraja C. ;

Rethore, Gildas ;

Monaghan, Michael ;

Fitzgerald, Kathleen ;

Gallagher, William ;

Pandit, Abhay .

BIOMATERIALS, 2010, 31 (32) :8188-8197

[5] Modeling the Time Evolution of the Nanoparticle-Protein Corona in a Body Fluid [J].

Dell'Orco, Daniele ;

Lundqvist, Martin ;

Oslakovic, Cecilia ;

Cedervall, Tommy ;

Linse, Sara .

PLOS ONE, 2010, 5 (06)

[6] Method for analysis of nanoparticle hemolytic properties in vitro [J].

Dobrovoiskaia, Marina A. ;

Clogston, Jeffrey D. ;

Neun, Barry W. ;

Hall, Jennifer B. ;

Patri, Anil K. ;

McNeil, Scott E. .

NANO LETTERS, 2008, 8 (08) :2180-2187

[7] Influence of surface charge density on protein adsorption on polymeric nanoparticles:: analysis by two-dimensional electrophoresis [J].

Gessner, A ;

Lieske, A ;

Paulke, BR ;

Müller, RH .

EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS, 2002, 54 (02) :165-170

[8]

Kang SC, 2007, J NANOSCI NANOTECHNO, V7, P3706, DOI 10.1166/jnn.2007.022

[9] INTERACTION BETWEEN PROTEINS AND LATEX-PARTICLES HAVING DIFFERENT SURFACE-STRUCTURES [J].

KAWAGUCHI, H ;

AMAGASA, H ;

HAGIYA, T ;

KIMURA, N ;

OHTSUKA, Y .

COLLOIDS AND SURFACES, 1985, 13 (04) :295-311

[10] EFFECT OF THE NATURE OF THE SUBSTRATE ON THE ADSORPTION OF HUMAN-PLASMA ALBUMIN [J].

KOUTSOUKOS, PG ;

MUMMEYOUNG, CA ;

NORDE, W ;

LYKLEMA, J .

COLLOIDS AND SURFACES, 1982, 5 (02) :93-104

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