Isolation of a novel microalgae strain Desmodesmus sp and optimization of environmental factors for its biomass production

被引:36
作者
Ji, Fang [1 ]
Hao, Rui [2 ]
Liu, Ying [3 ]
Li, Gang [4 ]
Zhou, Yuguang [1 ]
Dong, Renjie [1 ]
机构
[1] China Agr Univ, Coll Engn, Biomass Engn Ctr, Beijing, Peoples R China
[2] China Agr Univ, Coll Food Sci & Nutr Engn, Beijing, Peoples R China
[3] China Agr Univ, Coll Agr & Biotechnol, Beijing, Peoples R China
[4] China Agr Univ, Coll Water Resources & Civil Engn, Beijing, Peoples R China
基金
中国博士后科学基金;
关键词
Desmodesmus sp; Biomass production; Environmental factors; Response surface methodology; RESPONSE-SURFACE METHODOLOGY; WASTE-WATER; LIGHT-INTENSITY; LIPID-CONTENT; GROWTH; CULTIVATION; BIODIESEL; ALGAE; OIL; CYANOBACTERIA;
D O I
10.1016/j.biortech.2013.08.110
中图分类号
S2 [农业工程];
学科分类号
0828 ;
摘要
A novel strain of unicellular green algae was isolated from fresh water samples collected from Yesanpo National Geopark, Laishui County of Hebei Province, China. The morphological and genomic identification of this strain was carried out using 18 s rRNA analysis. This novel strain was identified as Desmodesmus sp. named as EJ15-2. Environmental factors for biomass production of Desmodesmus sp. EJ15-2 grown under autotrophic condition (BG11 medium) was optimized using response surface methodology (RSM). A high correlation coefficient (R-2 = 0.923, p <= 0.01) indicated the adaptability of the second-order equation matched well with the growth condition of this strain. The optimal conditions for a relatively high biomass production (up to 0.758 g/L) were at 30 degrees C, 98 mu mol/m(2)/s and 14:10 (LD), respectively. (C) 2013 Elsevier Ltd. All rights reserved.
引用
收藏
页码:249 / 254
页数:6
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