Fast-pulsing NMR techniques for the detection of weak interactions: successful natural abundance probe of hydrogen bonds in peptides

被引：7

作者：

Altmayer-Henzien, Amandine ^{[1
]}

Declerck, Valerie ^{[1
]}

Aitken, David J. ^{[1
]}

Lescop, Ewen ^{[2
]}

Merlet, Denis ^{[3
]}

Farjon, Jonathan ^{[3
]}

机构：

[1] Univ Paris 11, ICMMO UMR 8182, ESOM, F-91405 Orsay, France

[2] CNRS, Ctr Rech Gif, Inst Chim Subst Nat, F-91190 Gif Sur Yvette, France

[3] Univ Paris 11, ICMMO UMR 8182, ERMN, F-91405 Orsay, France

来源：

ORGANIC & BIOMOLECULAR CHEMISTRY | 2013年 / 11卷 / 43期

关键词：

FOLDAMERS; BETA; OLIGOMERS; PROTEINS; PULSES;

D O I：

10.1039/c3ob41876f

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Structural investigations of peptides using NMR spectroscopy rarely include the detection of N-H center dot center dot center dot O=C and N-H center dot center dot center dot N hydrogen bonds, because the relevant heteronuclei have a low natural abundance while the small trans hydrogen bond scalar couplings reduce the sensitivity. Fast repetition NMR techniques combined with state of the art spectrometer specifications allowed the enhancement of the sensitivity for detection of hydrogen bonds at natural isotopic abundance.

引用

页码：7611 / 7615

页数：5

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