Analysis of pectin from Panax ginseng flower buds and their binding activities to galectin-3

被引：56

作者：

Cui, Liangnan ^{[1
]}

Wang, Jiayi ^{[1
]}

Huang, Rui ^{[1
]}

Tan, Ya ^{[1
]}

Zhang, Fan ^{[1
]}

Zhou, Yifa ^{[1
]}

Sun, Lin ^{[1
]}

机构：

[1] Northeast Normal Univ, Sch Life Sci, Jilin Prov Key Lab Chem & Biol Nat Drugs Changbai, Changchun 130024, Jilin, Peoples R China

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2019年 / 128卷

基金：

中国国家自然科学基金;

关键词：

Ginseng flower buds; Polysaccharides; Pectin; Galectin-3; RHAMNOGALACTURONAN-II; POLYSACCHARIDE; LEAVES; MACROPHAGE; COMPONENTS; EXTRACTION; DOMAINS; FRUITS; MEYER; KDO;

D O I：

10.1016/j.ijbiomac.2019.01.129

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Water-soluble pectic polysaccharides isolated from Panax ginseng flower buds (WGFPA) were completely fractionated into six homogeneous fractions (WGFPA-1a, WGFPA-2a, WGFPA-3a, WGFPA-1b, WGFPA-2b and WGFPA-3b) by a combination of ion-exchange and size exclusion chromatographies. Monosaccharide composition, enzymatic hydrolysis and C-13 nuclear magnetic resonance (NMR) spectra analysis were combined to characterize their structural features. Furthermore, the interactions between these polysaccharides and galectin-3 were evaluated by biolayer interferometry assay. The results showed that WGFPA-1 a, WGFPA-2a and WGFPA-3a were rhamnogalacturonan I (RG-I) type pectin with abundant side chains, including alpha-L-1,5-arabinan,beta-D-1,4-galactan, arabinogalactan I (AG-I) and arabinogalactan II (AG-II), exhibiting strong binding activities to galectin-3 with apparent KD values 4.9 mu M, 0.71 mu M and 0.24 mu M, respectively. WGFPA-1b, WGFPA-2b and WGFPA-3b were homogalacturonan (HG) type pectin covalently linked with different ratios of rhamnogalacturonan II (RG-II) domains, showing weaker or no interactions with galectin-3. This study provides useful structural information for further investigation on the structure-activity relationship of ginseng flower buds pectin. (C) 2019 Elsevier B.V. All rights reserved.

引用

页码：459 / 467

页数：9