Evolution of Chloroplast J Proteins

被引:28
作者
Chiu, Chi-Chou [1 ,2 ]
Chen, Lih-Jen [1 ]
Su, Pai-Hsiang [1 ]
Li, Hsou-Min [1 ]
机构
[1] Acad Sinica, Inst Mol Biol, Taipei, Taiwan
[2] Natl Cheng Kung Univ, Inst Trop Plant Sci, Tainan 70101, Taiwan
来源
PLOS ONE | 2013年 / 8卷 / 07期
关键词
DEHYDROGENASE-LIKE COMPLEX; ESCHERICHIA-COLI DNAJ; HEAT-SHOCK PROTEINS; J-DOMAIN PROTEIN; SP PCC 6803; ARABIDOPSIS-THALIANA; MOLECULAR CHAPERONE; CHLAMYDOMONAS-REINHARDTII; HSP70; IMPORT;
D O I
10.1371/journal.pone.0070384
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Hsp70 chaperones are involved in multiple biological processes and are recruited to specific processes by designated J domain-containing cochaperones, or J proteins. To understand the evolution and functions of chloroplast Hsp70s and J proteins, we identified the Arabidopsis chloroplast J protein constituency using a combination of genomic and proteomic database searches and individual protein import assays. We show that Arabidopsis chloroplasts have at least 19 J proteins, the highest number of confirmed J proteins for any organelle. These 19 J proteins are classified into 11 clades, for which cyanobacteria and glaucophytes only have homologs for one clade, green algae have an additional three clades, and all the other 7 clades are specific to land plants. Each clade also possesses a clade-specific novel motif that is likely used to interact with different client proteins. Gene expression analyses indicate that most land plant-specific J proteins show highly variable expression in different tissues and are down regulated by low temperatures. These results show that duplication of chloroplast Hsp70 in land plants is accompanied by more than doubling of the number of its J protein cochaperones through adding new J proteins with novel motifs, not through duplications within existing families. These new J proteins likely recruit chloroplast Hsp70 to perform tissue specific functions related to biosynthesis rather than to stress resistance.
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页数:16
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