Diffusion of reagents in macrobeads

被引:29
作者
Groth, T [1 ]
Grotli, M [1 ]
Meldal, M [1 ]
机构
[1] Carlsberg Lab, Dept Chem, Ctr Solid Phase Organ Combinatorial Chem, DK-2500 Valby, Denmark
来源
JOURNAL OF COMBINATORIAL CHEMISTRY | 2001年 / 3卷 / 05期
关键词
D O I
10.1021/cc000106q
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
A simple and effective method of measuring diffusion rates of various acylating reagents in macro beads (in this work diameters greater than 570 mum) of amino-functionalized resins is presented. The extent of diffusion at various points of time was determined by treating beads with a staining reagent ("chloranil", 2,3,5,6-tetrachloro-1,4-benzoquinone) that colored the regions of the beads that had not yet been permeated by reagent (the central part of the beads). The volume of unstained resin (permeated part) was compared with the volume of the stained part, and diffusion rate constants were calculated. Factors influencing diffusion such as reagent concentration, solvent, temperature, resin type, and mechanical effects were investigated. The results showed that diffusion was promoted by increased temperature, good swelling of the resin, small reagents, and high concentrations but not by sonication or mechanical agitation.
引用
收藏
页码:461 / 468
页数:8
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