Synthesis of fucosylated lacto-N-tetraose using whole-cell biotransformation

被引：47

作者：

Baumgaertner, Florian ^{[1
]}

Jurzitza, Lukas ^{[1
]}

Conrad, Jurgen ^{[2
]}

Beifuss, Uwe ^{[2
]}

Sprenger, Georg A. ^{[1
]}

Albermann, Christoph ^{[1
]}

机构：

[1] Univ Stuttgart, Inst Microbiol, D-70569 Stuttgart, Germany

[2] Univ Hohenheim, Inst Chem, Bioorgan Chem, D-70599 Stuttgart, Germany

来源：

BIOORGANIC & MEDICINAL CHEMISTRY | 2015年 / 23卷 / 21期

关键词：

Biotransformation; Human milk oligosaccharides; Fucosyltransferase; Lacto-N-difucohexaose II; Lacto-N-fucopentaose I; Lacto-N-tetraose; HUMAN-MILK OLIGOSACCHARIDES; HELICOBACTER-PYLORI ALPHA-1,2-FUCOSYL-TRANSFERASE; ESCHERICHIA-COLI K-12; LARGE-SCALE SYNTHESIS; ENZYMATIC-SYNTHESIS; MOLECULAR-CLONING; EXPRESSION; BIOSYNTHESIS; RECOMBINANT; 2'-FUCOSYLLACTOSE;

D O I：

10.1016/j.bmc.2015.10.005

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Fucosylated oligosaccharides present a predominant group of free oligosaccharides found in human milk. Here, a microbial conversion of lactose, D-glucose and L-fucose to fucosylated lacto-N-tetraose by growing Escherichia coli cultures is presented. The recombinant expression of genes encoding for the beta 1,3-N-acetylglucosaminyltransferase (LgtA) and the beta 1,3-galactosyltransferase (WbgO) enables the whole-cell biotransformation of lactose to lacto-N-tetraose. By the additional expression of a recombinant GDP-L-fucose salvage pathway together with a bacterial fucosyltransferase, lacto-N-tetraose is further converted into the respective fucosylated compounds. The expression of a gene encoding the alpha 1,2-fucosyltransferase (FutC) in the lacto-N-tetraose producing E. coli strain led to the formation of lacto-N-fucopentaose I, whereas the expression of a gene encoding the oil,4-fucosyltransferase (FucT14) mainly led to the conversion of lacto-N-tetraose to lacto-N-difucohexaose II. (C) 2015 Elsevier Ltd. All rights reserved.

引用

页码：6799 / 6806

页数：8

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