Isolation of an eclosion hormone gene from the cotton bollworm, Helicoverpa armigera:: Temporal and spatial distribution of transcripts

被引:10
作者
Zhang, M
Xu, WH [1 ]
机构
[1] Univ Sci & Technol China, Sch Life Sci, Dept Mol & Cell Biol, Hefei 230027, Peoples R China
[2] Zhongshan Univ, Sch Life Sci, Inst Entomol, Guangzhou 510275, Peoples R China
[3] Zhongshan Univ, State Key Lab Biocontrol, Guangzhou 510275, Peoples R China
来源
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 2006年 / 143卷 / 03期
关键词
cDNA cloning; developmental expression; eclosion hormone; ecdysis; neurosecretory cells; immunoreactivity; Western blot analysis; Helicoverpa armigera;
D O I
10.1016/j.cbpb.2005.12.007
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The cDNA encoding eclosion hormone (EH), which plays an integral role in triggering ecdysis behavior at the end of each molt, was cloned from the cotton bollworm, Helicoverpa armigera (Har) (Lepidoptera: Noctuidae). The EH polyprotein precursor contains a 26-amino acid signal peptide and a single 62-amino acid mature EH. Compared the mature Har-EH with other known EHs, it shows 94%, 84%, and 59% identities to Manduca sexta, Bombyx mori, and Drosophila melanogaster, respectively. Har-EH mRNA is expressed only in the brain by Northern blot and RT-PCR, but not in other tissues. By in situ hybridization and immunocytochemistry, both Har-EH mRNA and protein are localized in two pairs of neurosecretory cells of the brain. Prior to a molt, expression level of Har-EH gene reaches the highest point, and then drops after molt. EH release is detected both centrally, within the ganglia, and peripherally, into the hemolymph. A peak of the EH titer in hemolymph measured by ELISA presents at ecdysis. These results are consistent with the biological function of Har-EH associated with ecdysis. Furthermore, Har-EH gene is expressed throughout all of the developmental stages examined, implicating that the EH gene may possess other biological functions in post-embryonic development other than triggering ecdysis behavior. (C) 2005 Elsevier Inc. All rights reserved.
引用
收藏
页码:351 / 359
页数:9
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