CD3+CD56+T Lymphocytes Are Associated With ER Stress and Inflammasome Activation in Type 1 Diabetes

被引：4
作者：
Huang, Yu-nan [1 ,2 ]
Lin, Wei-de [3 ,4 ]
Su, Pen-hua [5 ,6 ]
Bau, Da-tian [7 ,8 ]
Tsai, Fuu-jen [2 ,3 ]
Huang, Chieh-chen [1 ,10 ]
Wang, Chung-hsing [2 ,9 ,11 ]
机构：
[1] Natl Chung Hsing Univ, Dept Life Sci, Taichung, Taiwan
[2] Childrens Hosp China Med Univ, Div Genet & Metab, Taichung, Taiwan
[3] China Med Univ Hosp, Genet Ctr, Dept Med Res, Taichung, Taiwan
[4] China Med Univ, Sch Post Baccalaureate Chinese Med, Taichung, Taiwan
[5] Chung Shan Med Univ Hosp, Dept Pediat, Taichung, Taiwan
[6] Chung Shan Med Univ, Sch Med, Taichung, Taiwan
[7] China Med Univ Hosp, Dept Med Res, Terry Fox Canc Res Lab, Taichung, Taiwan
[8] China Med Univ, Grad Inst Biomed Sci, Taichung, Taiwan
[9] China Med Univ, Sch Med, Taichung, Taiwan
[10] Natl Chung Hsing Univ, Dept Life Sci, Taichung 404, Taiwan
[11] Childrens Hosp China Med Univ, Div Genet & Metab, 2 Yuh Der Rd, Taichung 404, Taiwan
来源：
IN VIVO | 2022年 / 36卷 / 05期
关键词：
Type; 1; diabetes; endoplasmic reticulum; inflammasome activation; ENDOPLASMIC-RETICULUM STRESS; KILLER T-CELLS; UNFOLDED PROTEIN RESPONSE; PROGRESSION; MICE;
D O I：
10.21873/invivo.12934
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
Background/Aim: The T cell's flexibility of the immune system to be regulated affects the onset of type 1 diabetes (T1D). However, the mechanisms of endoplasmic reticulum (ER) stress and inflammasome activation in the circulating CD3+CD56+ T cells of patients with T1D remain unclear. This study evaluated the role of CD3+CD56+ T cells in T1D and their correlations with ER stress, inflammasome activation and disease characteristics. Materials and Methods: The frequency of circulating CD3+CD56+ T cells was determined using flow cytometry in healthy individuals and patients with T1D. Calnexin, NLR family pyrin domain containing 3 (NLRP3), ASC, caspase-1 (Casp1), cleaved caspase-3 (C-Casp3), and annexin V (AnnV) expression and propidium iodide staining in CD3+/CD56+ T cells were analyzed using flow cytometry. Results: The frequency of CD3+CD56+ T cells was reduced in patients with T1D relative to that in healthy individuals. In addition, high calnexin, NLRP3, ASC and Casp1 expression in CD3+CD56+ T cells was negatively correlated with the percentage of CD3+CD56+ T cells in patients with T1D. Conclusion: ER stress, inflammasome activation, and a lower peripheral frequency of circulating CD3+CD56+ T cells might indicate disease progression and necessitate clinical T1D immunological self-tolerance monitoring.
引用
收藏
页码：2083 / 2091
页数：9
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