Establishment of a reverse transcription recombinase-aided amplification detection method for porcine group a rotavirus

被引：6

作者：

Wang, Yushun ^{[1
]}

Nie, Mincai ^{[1
]}

Deng, Huidan ^{[1
]}

Lai, Siyuan ^{[1
]}

Zhou, Yuancheng ^{[2
,3
]}

Sun, Xiangan ^{[1
]}

Zhu, Ling ^{[1
,4
]}

Xu, Zhiwen ^{[1
,4
]}

机构：

[1] Sichuan Agr Univ, Coll Vet Med, Chengdu, Peoples R China

[2] Sichuan Anim Sci Acad, Anim Breeding & Genet Key Lab Sichuan Prov, Chengdu, Peoples R China

[3] Sichuan Anim Sci Acad, Livestock & Poultry Biol Prod Key Lab Sichuan Prov, Chengdu, Peoples R China

[4] Sichuan Agr Univ, Coll Vet Med, Sichuan Key Lab Anim Epidem Dis & Human Hlth, Chengdu, Peoples R China

来源：

FRONTIERS IN VETERINARY SCIENCE | 2022年 / 9卷

关键词：

porcine rotavirus; VP6; gene; RT-RAA; RT-qPCR methods; detection assay; RAPID DETECTION; MOLECULAR CHARACTERIZATION; ASSAY; GENE; DIARRHEA;

D O I：

10.3389/fvets.2022.954657

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Porcine rotavirus type A (PoRVA) is the main cause of dehydration and diarrhea in piglets, which has a great impact on the development of the pig industry worldwide. A rapid, accurate and sensitive detection method is conducive to the monitoring, control, and removal of PoRVA. In this study, a PoRVA real-time fluorescent reverse transcription recombinase-aided amplification (RT-RAA) assay was developed. Based on the PoRVA VP6 gene, specific primers and probes were designed and synthesized. The sensitivity of RT-RAA and TaqMan probe-based RT-qPCR was 7 copies per reaction and 5 copies per reaction, respectively. The sensitivity of the RT-RAA method was close to TaqMan probe-based RT-qPCR. The detection results of RT-RAA and TaqMan probe-based quantitative real-time RT-PCR methods were completely consistent in 241 clinical samples. Therefore, we successfully established a rapid and specific RT-RAA diagnostic method for PoRVA.

引用

页数：6

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