Establishment of a reverse transcription recombinase-aided amplification detection method for porcine group a rotavirus

被引：6
作者：
Wang, Yushun [1 ]
Nie, Mincai [1 ]
Deng, Huidan [1 ]
Lai, Siyuan [1 ]
Zhou, Yuancheng [2 ,3 ]
Sun, Xiangan [1 ]
Zhu, Ling [1 ,4 ]
Xu, Zhiwen [1 ,4 ]
机构：
[1] Sichuan Agr Univ, Coll Vet Med, Chengdu, Peoples R China
[2] Sichuan Anim Sci Acad, Anim Breeding & Genet Key Lab Sichuan Prov, Chengdu, Peoples R China
[3] Sichuan Anim Sci Acad, Livestock & Poultry Biol Prod Key Lab Sichuan Prov, Chengdu, Peoples R China
[4] Sichuan Agr Univ, Coll Vet Med, Sichuan Key Lab Anim Epidem Dis & Human Hlth, Chengdu, Peoples R China
来源：
FRONTIERS IN VETERINARY SCIENCE | 2022年 / 9卷
关键词：
porcine rotavirus; VP6; gene; RT-RAA; RT-qPCR methods; detection assay; RAPID DETECTION; MOLECULAR CHARACTERIZATION; ASSAY; GENE; DIARRHEA;
D O I：
10.3389/fvets.2022.954657
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
Porcine rotavirus type A (PoRVA) is the main cause of dehydration and diarrhea in piglets, which has a great impact on the development of the pig industry worldwide. A rapid, accurate and sensitive detection method is conducive to the monitoring, control, and removal of PoRVA. In this study, a PoRVA real-time fluorescent reverse transcription recombinase-aided amplification (RT-RAA) assay was developed. Based on the PoRVA VP6 gene, specific primers and probes were designed and synthesized. The sensitivity of RT-RAA and TaqMan probe-based RT-qPCR was 7 copies per reaction and 5 copies per reaction, respectively. The sensitivity of the RT-RAA method was close to TaqMan probe-based RT-qPCR. The detection results of RT-RAA and TaqMan probe-based quantitative real-time RT-PCR methods were completely consistent in 241 clinical samples. Therefore, we successfully established a rapid and specific RT-RAA diagnostic method for PoRVA.
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页数：6
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