Lactoperoxidase immobilization on silver nanoparticles enhances its antimicrobial activity

被引：12

作者：

Sheikh, Ishfaq Ahmad ^{[1
]}

Yasir, Muhammad ^{[1
]}

Khan, Imran ^{[1
]}

Khan, Sher Bahadur ^{[2
]}

Azum, Naveed ^{[2
]}

Jiffri, Essam Hussain ^{[3
]}

Kamal, Mohammad Amjad ^{[1
]}

Ashraf, Ghulam Md ^{[1
]}

Beg, Mohd Amin ^{[1
]}

机构：

[1] King Abdulaziz Univ, King Fahd Med Res Ctr, Jeddah, Saudi Arabia

[2] King Abdulaziz Univ, Dept Chem, Ctr Excellence Adv Mat Res, Fac Sci, Jeddah, Saudi Arabia

[3] King Abdulaziz Univ, Fac Appl Med Sci, Jeddah, Saudi Arabia

来源：

JOURNAL OF DAIRY RESEARCH | 2018年 / 85卷 / 04期

关键词：

Antimicrobial activity; immobilization; lactoperoxidase; silver nanoparticles; INACTIVATION; STABILITY; MECHANISM; PEPTIDE; MILK;

D O I：

10.1017/S0022029918000730

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

Lactoperoxidase (LPO) is an antimicrobial protein present in milk that plays an important role in natural defence mechanisms during neonatal and adult life. The antimicrobial activity of LPO has been commercially adapted for increasing the shelf life of dairy products. Immobilization of LPO on silver nanoparticles (AgNPs) is a promising way to enhance the antimicrobial activity of LPO. In the current study, LPO was immobilized on AgNPs to form LPO/AgNP conjugate. The immobilized LPO/AgNP conjugate was characterized by various biophysical techniques. The enhanced antibacterial activity of the conjugate was tested against E. coli in culture at 2 h intervals for 10 h. The results showed successful synthesis of spherical AgNPs. LPO was immobilized on AgNPs with agglomerate sizes averaging approximately 50 nm. The immobilized conjugate exhibited stronger antibacterial activity against E. coli in comparison to free LPO. This study may help in increasing the efficiency of lactoperoxidase system and will assist in identifying novel avenues to enhance the stability and antimicrobial function of LPO system in dairy and other industries.

引用

页码：460 / 464

页数：5

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