Inactivated infectious haematopoietic necrosis virus (IHNV) vaccines

被引：57

作者：

Anderson, E. ^{[1
]}

Clouthier, S. ^{[1
]}

Shewmaker, W. ^{[2
]}

Weighall, A. ^{[2
]}

LaPatra, S. ^{[2
]}

机构：

[1] Maine BioTek Inc, Winterport, ME 04496 USA

[2] Clear Springs Foods Inc, Buhl, ID USA

来源：

JOURNAL OF FISH DISEASES | 2008年 / 31卷 / 10期

关键词：

inactivated vaccine; infectious haematopoietic necrosis virus; Oncorhynchus mykiss; rainbow trout;

D O I：

10.1111/j.1365-2761.2008.00960.x

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

The inactivation dynamics of infectious haematopoietic necrosis vials (IHNV) by beta-propiolactone (BPL), binary ethylenimine (BEI), formaldehyde or heat and the antigenic and immunogenic properties of the inactivated vaccines were evaluated. Chemical treatment of IHNV with 2.7 trim BPL, 1.5 mm BEI or 50 mm formaldehyde abolished virus infectivity within 48 h whereas heat treatment at 50 or 100 degrees C tendered the virus Innocuous within 30 min. The inactivated IHNV vaccines were recognized by rainbow trout, Oncorhynchus mykiss, IHNV-specific antibodies and were differentially recognized by antigenic site I or antigenic site 11 IHNV glycoprotein-specific neutralizing monoclonal antibodies. The BPL inactivated whole virus vaccine was highly efficacious in vaccinated rainbow trout challenged by waterborne exposure to IHNV 7, 28, 42 or 56 days (15 degrees C) after immunization. The formaldehyde inactivated whole virus vaccine was efficacious 7 or 11 days after vaccination of rainbow trout but performed inconsistently when tested at later time points. The other vaccines tested were not efficacious.

引用

页码：729 / 745

页数：17

共 67 条

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