Development and mapping of a public reference set of SSR markers in Lolium perenne L.

被引:49
作者
Jensen, LB [1 ]
Muylle, H
Arens, P
Andersen, CH
Holm, PB
Ghesquiere, M
Julier, B
Lübberstedt, T
Nielsen, KK
De Riek, J
Roldán-Ruiz, I
Roulund, N
Taylor, C
Vosman, B
Barre, P
机构
[1] Res Ctr Flakkebjerg, DIAS, Dept Genet & Biotechnol, DK-4200 Slagelse, Denmark
[2] Dept Plant Genet & Breeding DvP, B-9090 Melle, Belgium
[3] Dansk Planteforaedling, DLF Trifolium, DK-4660 Store Heddinge, Denmark
[4] INRA, UGAPF, F-86600 Lusignan, France
[5] PRI, NL-6700 AA Wageningen, Netherlands
来源
MOLECULAR ECOLOGY NOTES | 2005年 / 5卷 / 04期
关键词
consensus linkage map; reference set; ryegrass; SSR;
D O I
10.1111/j.1471-8286.2005.01043.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We report on the characterization and mapping of 76 simple sequence repeat (SSR) markers for Lolium perenne. These markers are publicly available or obtained either from genomic libraries enriched for SSR motifs or L. perenne expressed sequence tag (EST) clones. Four L. perenne mapping populations were used to map the SSR markers. A consensus linkage map of the four mapping populations containing 65 of the SSR markers is presented, together with primer information and a quality score indicating the usefulness of the SSR marker in different populations. The SSR markers identified all seven L. perenne linkage groups.
引用
收藏
页码:951 / 957
页数:7
相关论文
共 18 条
[1]  
BARRE P, 2000, 2 MOL BREED FOR CROP, P60
[2]   A high-density molecular map for ryegrass (Lolium perenne) using AFLP markers [J].
Bert, PF ;
Charmet, G ;
Sourdille, P ;
Hayward, MD ;
Balfourier, F .
THEORETICAL AND APPLIED GENETICS, 1999, 99 (3-4) :445-452
[3]   M13-tailed primers improve the readability and usability of microsatellite analyses performed with two different allele-sizing methods [J].
Boutin-Ganache, I ;
Raposo, M ;
Raymond, M ;
Deschepper, CF .
BIOTECHNIQUES, 2001, 31 (01) :24-+
[4]   Microsatellite enrichment in organisms with large genomes (Allium cepa L.) [J].
Fischer, D ;
Bachmann, K .
BIOTECHNIQUES, 1998, 24 (05) :796-+
[5]   CLONING AND CHARACTERIZATION OF A NEW RYE-SPECIFIC REPEATED SEQUENCE [J].
GUIDET, F ;
ROGOWSKY, P ;
TAYLOR, C ;
SONG, W ;
LANGRIDGE, P .
GENOME, 1991, 34 (01) :81-87
[6]   QTL mapping of vernalization response in perennial ryegrass (Lolium perenne L.) reveals co-location with an orthologue of wheat VRN1 [J].
Jensen, LB ;
Andersen, JR ;
Frei, U ;
Xing, YZ ;
Taylor, C ;
Holm, PB ;
Lübberstedt, TL .
THEORETICAL AND APPLIED GENETICS, 2005, 110 (03) :527-536
[7]   Development and characterisation of simple sequence repeat (SSR) markers for perennial ryegrass (Lolium perenne L.) [J].
Jones, ES ;
Dupal, MP ;
Kölliker, R ;
Drayton, MC ;
Forster, JW .
THEORETICAL AND APPLIED GENETICS, 2001, 102 (2-3) :405-415
[8]   Genetic diversity in seven perennial Ryegrass (Lolium perenne L.) cultivars based on SSR markers [J].
Kubik, C ;
Sawkins, M ;
Meyer, WA ;
Gaut, BS .
CROP SCIENCE, 2001, 41 (05) :1565-1572
[9]   Sixty simple sequence repeat markers for use in the Festuca-Lolium complex of grasses [J].
Lauvergeat, V ;
Barre, P ;
Bonnet, M ;
Ghesquière, M .
MOLECULAR ECOLOGY NOTES, 2005, 5 (02) :401-405
[10]  
MUYLLE H, 2003, THESIS U GHENT BELGI