Quantitative variations in the vaginal bacterial population associated with asymptomatic infections: a real-time polymerase chain reaction study

被引:51
作者
Biagi, E. [1 ]
Vitali, B. [1 ]
Pugliese, C. [1 ]
Candela, M. [1 ]
Donders, G. G. G. [2 ,3 ,4 ]
Brigidi, P. [1 ]
机构
[1] Univ Bologna, Dept Pharmaceut Sci, I-40126 Bologna, Italy
[2] Femicare Clin Res Women, Tienen, Belgium
[3] Catholic Univ Louvain, Dept Obstet & Gynaecol, Gen Hosp Heilig Hart Tienen, B-3000 Louvain, Belgium
[4] Catholic Univ Louvain, Univ Hosp Gasthuisberg, B-3000 Louvain, Belgium
关键词
GROUP-SPECIFIC PRIMERS; GARDNERELLA-VAGINALIS; PREDOMINANT BACTERIA; LACTOBACILLUS SPP; MYCOPLASMA-HOMINIS; HUMAN FECES; VAGINOSIS; PCR; WOMEN; CANDIDIASIS;
D O I
10.1007/s10096-008-0617-0
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
The real-time polymerase chain reaction (PCR) quantification of several vaginal bacterial groups in healthy women and patients developing asymptomatic bacterial vaginosis (BV) and candidiasis (CA) was performed. Statistical analysis revealed that the BV condition is characterised by a great variability among subjects and that it is associated with a significant increase of Prevotella, Atopobium, Veillonella and Gardnerella vaginalis, and a drop in Lactobacillus. On the contrary, the vaginal microflora of healthy women and patients developing CA was found to be homogeneous and stable over time.
引用
收藏
页码:281 / 285
页数:5
相关论文
共 16 条
  • [1] Quantitative determination by real-time PCR of four vaginal Lactobacillus species, Gardnerella vaginalis and Atopobium vaginae indicates an inverse relationship between L. gasseri and L. iners
    De Backer, Ellen
    Verhelst, Rita
    Verstraelen, Hans
    Alqumber, Mohammed A.
    Burton, Jeremy P.
    Tagg, John R.
    Temmerman, Marleen
    Vaneechoutte, Mario
    [J]. BMC MICROBIOLOGY, 2007, 7 (1)
  • [2] HISTORY AND REVIEW OF BACTERIAL VAGINOSIS
    ESCHENBACH, DA
    [J]. AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY, 1993, 169 (02) : 441 - 445
  • [3] Molecular identification of bacteria associated with bacterial vaginosis
    Fredricks, DN
    Fiedler, TL
    Marrazzo, JM
    [J]. NEW ENGLAND JOURNAL OF MEDICINE, 2005, 353 (18) : 1899 - 1911
  • [4] Asymptomatic bacterial vaginosis: is it time to treat?
    Gibbs, Ronald S.
    [J]. AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY, 2007, 196 (06) : 495 - 496
  • [5] Molecular diversity of Lactobacillus spp. and other lactic acid bacteria in the human intestine as determined by specific amplification of 16S ribosomal DNA
    Heilig, HGHJ
    Zoetendal, EG
    Vaughan, EE
    Marteau, P
    Akkermans, ADL
    de Vos, WM
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2002, 68 (01) : 114 - 123
  • [6] Microbes on the human vaginal epithelium
    Hyman, RW
    Fukushima, M
    Diamond, L
    Kumm, J
    Giudice, LC
    Davis, RW
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (22) : 7952 - 7957
  • [7] Use of 16S rRNA gene-targeted group-specific primers for real-time PCR analysis of predominant bacteria in human feces
    Matsuki, T
    Watanabe, K
    Fujimoto, J
    Takada, T
    Tanaka, R
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2004, 70 (12) : 7220 - 7228
  • [8] Development of 16S rRNA-gene-targeted group-specific primers for the detection and identification of predominant bacteria in human feces
    Matsuki, T
    Watanabe, K
    Fujimoto, J
    Miyamoto, Y
    Takada, T
    Matsumoto, K
    Oyaizu, H
    Tanaka, R
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2002, 68 (11) : 5445 - 5451
  • [9] Development of an extensive set of 16S rDNA-targeted primers for quantification of pathogenic and indigenous bacteria in faecal samples by real-time PCR
    Rinttilä, T
    Kassinen, A
    Malinen, E
    Krogius, L
    Palva, A
    [J]. JOURNAL OF APPLIED MICROBIOLOGY, 2004, 97 (06) : 1166 - 1177
  • [10] Lactobacilli in the female genital tract in relation to other genital microbes and vaginal pH
    Roennqvist, Per Daniel Johannes
    Forsgren-Brusk, Ulla Birgitta
    Hakansson, Eva Elisabeth Grahn
    [J]. ACTA OBSTETRICIA ET GYNECOLOGICA SCANDINAVICA, 2006, 85 (06) : 726 - 735