Response surface optimization for the production of marine eubacterial protease and its application

被引：0

作者：

Richa, K. ^{[1
]}

Bose, H. ^{[1
]}

Singh, K. ^{[1
]}

Karthik, L. ^{[1
]}

Kumar, G. ^{[1
]}

Rao, Bhaskara K. V. ^{[1
]}

机构：

[1] VIT Univ, Sch Bio Sci & Technol, Environm Biotechnol Div, Mol & Microbiol Res Lab, Vellore 632014, Tamil Nadu, India

来源：

RESEARCH JOURNAL OF BIOTECHNOLOGY | 2013年 / 8卷 / 04期

关键词：

Protease; Bacillus VITRKHB; Response surface methodology; DEAE ion - exchange chromatography; optimization; ALKALINE PROTEASE; BACILLUS-LICHENIFORMIS; PURIFICATION; CARBON;

D O I：

暂无

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Marine Bacillus VITRKHB showed substantial amount of proteolytic activity. The impact of different carbon and nitrogen source on protease yield and activity was investigated following its production. The maximum enzyme activity was observed in case of fructose as carbon and nutrient broth as nitrogen supplement. Statistical experimental designs using Response surface methodology (RSM) were employed to study the mutual interactive effect of major nutrients and physicochemical parameters influencing the enzyme production. Optimal level of different variables for maximum protease activity was determined as fructose 2.50%, nutrient broth 0.70%, pH 6.75, NaCl 4.75% and temperature 40 degrees C after 24h of incubation. Determination of co-efficient (R-2 = 0.8528) close to 1.0 indicated significant precision and reliability of the response data. Extracellular protease was isolated, purified to 1.19 fold by DEAE ion - exchange chromatography and its specific activity was determined to be 1.59 IU/mg. Industrial efficacy of the marine eubacterial protease was ascertained.

引用

页码：78 / 85

页数：8

共 40 条

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