Genetic and epigenetic stability of cryopreserved and cold-stored hops (Humulus lupulus L.)

被引：49
|
作者：
Peredo, Elena L. [1 ]
Arroyo-Garcia, Rosa [2 ]
Reed, Barbara M. [3 ]
Angeles Revilla, M. [1 ]
机构：
[1] Univ Oviedo, Dept Plant Physiol, E-33071 Oviedo, Spain
[2] Inst Nacl Invest Agr & Alimentaria, Dept Biotechnol, Madrid 28040, Spain
[3] ARS, USDA, Corvallis, OR 97333 USA
来源：
CRYOBIOLOGY | 2008年 / 57卷 / 03期
关键词：
AFLP; MSAP; RAPD; Somaclonal variation;
D O I：
10.1016/j.cryobiol.2008.09.002
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Conventional cold storage and cryopreservation methods for hops (Humulus lupulus L) are available but, to our knowledge, the genetic and epigenetic stability of the recovered plants have not been tested. This study analyzed 51 accessions of hop using the molecular techniques, Random Amplified DNA Polymorphism (RAPD) and Amplified Fragment Length Polymorphism (AFLP), revealing no genetic variation among green house-grown controls and cold stored or cryopreserved plants. Epigenetic stability was evaluated using Methylation Sensitive Amplified Polymorphism (MSAP). Over 36% of the loci were polymorphic when the cold and cryo-treated plants were compared to greenhouse plants. The main changes were demethylation events and they were common to the cryopreserved and cold stored plants indicating the possible effect of the in vitro establishment process, an essential step in both protocols. Protocol-specific methylation patterns were also detected indicating that both methods produced epigenetic changes in plants following cold storage and cryopreservation. (C) 2008 Elsevier Inc. All rights reserved.
引用
收藏
页码：234 / 241
页数：8
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