Bioluminescence imaging of leukemia cell lines in vitro and in mouse xenografts: effects of monoclonal and polyclonal cell populations on intensity and kinetics of photon emission

被引：23

作者：

Christoph, Sandra ^{[1
,2
]}

Schlegel, Jennifer ^{[1
]}

Alvarez-Calderon, Francesca ^{[3
,4
]}

Kim, Yong-Mi ^{[5
]}

Brandao, Luis N. ^{[1
]}

DeRyckere, Deborah ^{[1
]}

Graham, Douglas K. ^{[1
]}

机构：

[1] Univ Colorado Denver, Sch Med, Dept Pediat, Aurora, CO 80045 USA

[2] Univ Hosp Essen, Dept Bone Marrow Transplant, D-45122 Essen, Germany

[3] Natl Jewish Hlth, Integrated Dept Immunol, Denver, CO 80206 USA

[4] Univ Colorado Denver, Sch Med, Med Scientist Training Program, Aurora, CO 80045 USA

[5] Childrens Hosp Los Angeles, Los Angeles, CA 90027 USA

来源：

JOURNAL OF HEMATOLOGY & ONCOLOGY | 2013年 / 6卷

基金：

美国国家卫生研究院;

关键词：

Bioluminescence imaging; Monoclonal population; Polyclonal population; Firefly luciferase; Leukemia; Xenograft; GROWTH;

D O I：

10.1186/1756-8722-6-10

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Background: We investigated the utility of bioluminescence imaging (BLI) using firefly luciferase in monoclonal and polyclonal populations of leukemia cells in vitro and in vivo. Methods: Monoclonal and polyclonal human lymphoid and myeloid leukemia cell lines transduced with firefly luciferase were used for BLI. Results: Kinetics and dynamics of bioluminescence signal were cell line dependent. Luciferase expression decreased significantly over time in polyclonal leukemia cells in vitro. Transplantation of polyclonal luciferase-tagged cells in mice resulted in inconsistent signal intensity. After selection of monoclonal cell populations, luciferase activity was stable, equal kinetic and dynamic of bioluminescence intensity and strong correlation between cell number and light emission in vitro were observed. We obtained an equal development of leukemia burden detected by luciferase activity in NOD-scid-gamma mice after transplantation of monoclonal populations. Conclusion: The use of monoclonal leukemia cells selected for stable and equal luciferase activity is recommended for experiments in vitro and xenograft mouse models. The findings are highly significant for bioluminescence imaging focused on pre-clinical drug development.

引用

页数：9

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