Hemin-mediated hemolysis in erythrocytes: Effects of ascorbic acid and glutathione

被引：27

作者：

Li, SD

Su, YD

Li, M

Zou, CG ^{[1
]}

机构：

[1] Yunnan Univ, Sch Life Sci, Kunming 650091, Peoples R China

[2] Kunming Med Coll, Dept Biochem, Kunming 650031, Peoples R China

[3] Kunming Med Coll, Dept Clin Chem, Kunming 650031, Peoples R China

来源：

ACTA BIOCHIMICA ET BIOPHYSICA SINICA | 2006年 / 38卷 / 01期

关键词：

erythrocyte; ascorbic acid; hemin; glutathione; H2O2;

D O I：

10.1111/j.1745-7270.2006.00127.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In the present work, we investigated the effect of ascorbic acid and glutathione on hemolysis induced by hemin in erythrocytes. Ascorbic acid not only enhanced hemolysis, but also induced formation of thiobarbituric acid-reactive substances in the presence of hemin. It has been shown that glutathione inhibits hemin-induced hemolysis by mediating hemin degradation. Erythrocytes depleted of glutathione became very sensitive to oxidative stress induced by hemin and ascorbic acid. H2O2 was involved in hemin-mediated hemolysis in the presence of ascorbic acid. However, a combination of glutathione and ascorbic acid was more effective in inhibiting hemolysis induced by hemin than glutathione alone. Extracellular and intracellular ascorbic acid exhibited a similar effect on hemin-induced hemolysis or inhibition of hemin-induced hemolysis by glutathione. The current study indicates that ascorbic acid might function as an antioxidant or prooxidant in hemin-mediated hemolysis, depending on whether glutathione is available.

引用

页码：63 / 69

页数：7

共 30 条

[1] HEME DEGRADATION IN THE PRESENCE OF GLUTATHIONE - A PROPOSED MECHANISM TO ACCOUNT FOR THE HIGH-LEVELS OF NONHEME IRON FOUND IN THE MEMBRANES OF HEMOGLOBINOPATHIC RED-BLOOD-CELLS [J].