Novel method for screening Saccharomyces cerevisiae mutants with increased sulfur-containing compounds: Color-based selection of ade1 or ade2 mutants

被引：2
作者：
Tarutina, Marina G. [1 ]
Dutova, Tatiana A. [1 ]
Yezhova, Inna E. [1 ]
Nishiuchi, Hiroaki [2 ]
Sineoky, Sergey P. [1 ]
机构：
[1] State Res Inst Genet & Select Ind Microorganisms, Russian State Collect Ind Microorganisms VKPM, Moscow 117545, Russia
[2] Ajinomoto Co Inc, Kawasaki Ku, Kawasaki, Kanagawa 2108681, Japan
来源：
JOURNAL OF BIOSCIENCE AND BIOENGINEERING | 2012年 / 114卷 / 06期
关键词：
Visual selection of colonies; Glutathione; Yeast with red pigmentation; Saccharomyces cerevisiae; ade1; mutation; ade2; RED PIGMENT; YEAST; GLUTATHIONE; SYNTHETASE; PATHWAY; GENES;
D O I：
10.1016/j.jbiosc.2012.07.009
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
We identified Saccharomyces cerevisiae mutants with 100% higher intracellular glutathione using 1-methyl-3-nitro-1-nitrosoguanidine mutagenesis. This method employs visual selection of the most pigmented colonies among met30 strains carrying ade1 and ade2 mutations. Since the method does not involve genetic engineering, the mutants are suitable for use in the food industry. (C) 2012, The Society for Biotechnology, Japan. All rights reserved.
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页码：615 / 618
页数：4
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