Impedimetric biosensor based on cell-mediated bioimprinted films for bacterial detection

被引：86
作者：
Qi, Peng [1 ,2 ]
Wan, Yi [1 ,2 ]
Zhang, Dun [1 ]
机构：
[1] Chinese Acad Sci, Inst Oceanol, Shandong Prov Key Lab Corros Sci, Qingdao 266071, Peoples R China
[2] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
来源：
BIOSENSORS & BIOELECTRONICS | 2013年 / 39卷 / 01期
基金：
中国国家自然科学基金;
关键词：
Sulfate-reducing bacteria; Graphene; Electrochemical impedance spectroscopy; Bioimprinted film; Electrodeposition; SULFATE-REDUCING BACTERIA; MOLECULARLY IMPRINTED POLYMERS; GRAPHENE SHEETS; NANOPARTICLES; SURFACE; HYBRIDIZATION; FLUORESCENCE; VANCOMYCIN; PATHOGENS; ELECTRODE;
D O I：
10.1016/j.bios.2012.07.078
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
This work presents the synthesis of bacteria-mediated bioimprinted films for selective bacterial detection. Marine pathogen sulfate-reducing bacteria (SRB) were chosen as the template bacteria. Chitosan (CS) doped with reduced graphene sheets (RGSs) was electrodeposited on an indium tin oxide electrode, and the resulting RGSs-CS hybrid film served as a platform for bacterial attachment. The electrodeposition conditions were optimized to obtain RGSs-CS hybrid films with excellent electrochemical performance. A layer of nonconductive CS film was deposited to embed the pathogen, and acetone was used to wash away the bacterial templates. Electrochemical impedance spectroscopy was performed to characterize the stepwise modification process and monitor the SRB population. Faradic impedance measurements revealed that the charge transfer resistance (R-ct) increased with increased SRB concentration. A linear relationship between Delta R-ct and the logarithm of SRB concentration was obtained within the concentration range of 1.0 x 10(4) cfu mL(-1) to 1.0 x 10(8) cfu mL(-1). The impedimetric sensor showed good selectivity towards SRB based on size and shape. Hence, selectivity for bacterial detection can be improved if the bioimprinting technique is combined with other bio-recognition elements. (C) 2012 Elsevier B.V. All rights reserved.
引用
收藏
页码：282 / 288
页数：7
相关论文
共 48 条
[11]   Using biofunctional magnetic nanoparticles to capture vancomycin-resistant enterococci and other gram-positive bacteria at ultralow concentration [J].
Gu, HW ;
Ho, PL ;
Tsang, KWT ;
Wang, L ;
Xu, B .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (51) :15702-15703
[12]   Presenting vancomycin on nanoparticles to enhance antimicrobial activities [J].
Gu, HW ;
Ho, PL ;
Tong, E ;
Wang, L ;
Xu, B .
NANO LETTERS, 2003, 3 (09) :1261-1263
[13]   Molecularly imprinted polymers and their use in biomimetic sensors [J].
Haupt, K ;
Mosbach, K .
CHEMICAL REVIEWS, 2000, 100 (07) :2495-2504
[14]   Mass-sensitive detection of cells, viruses and enzymes with artificial receptors [J].
Hayden, O ;
Bindeus, R ;
Haderspöck, C ;
Mann, KJ ;
Wirl, B ;
Dickert, FL .
SENSORS AND ACTUATORS B-CHEMICAL, 2003, 91 (1-3) :316-319
[15]  
Hayden O, 2001, ADV MATER, V13, P1480, DOI 10.1002/1521-4095(200110)13:19<1480::AID-ADMA1480>3.0.CO
[16]  
2-V
[17]   Artificial antibodies for bioanalyte detection-sensing viruses and proteins [J].
Hayden, Oliver ;
Lieberzeit, Peter A. ;
Blaas, Dieter ;
Dickert, Franz L. .
ADVANCED FUNCTIONAL MATERIALS, 2006, 16 (10) :1269-1278
[18]   A disposable microfluidic biochip with on-chip molecularly imprinted biosensors for optical detection of anesthetic propofol [J].
Hong, Chien-Chong ;
Chang, Po-Hsiang ;
Lin, Chih-Chung ;
Hong, Chian-Lang .
BIOSENSORS & BIOELECTRONICS, 2010, 25 (09) :2058-2064
[19]   Green-synthesized gold nanoparticles decorated graphene sheets for label-free electrochemical impedance DNA hybridization biosensing [J].
Hu, Yuwei ;
Hua, Shucheng ;
Li, Fenghua ;
Jiang, Yuanyuan ;
Bai, Xiaoxue ;
Li, Dan ;
Niu, Li .
BIOSENSORS & BIOELECTRONICS, 2011, 26 (11) :4355-4361
[20]   PREPARATION OF GRAPHITIC OXIDE [J].
HUMMERS, WS ;
OFFEMAN, RE .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1958, 80 (06) :1339-1339
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