Photon-burst analysis in two-photon fluorescence excitation flow cytometry

被引：0

作者：

Hänninen, PE ^{[1
]}

Soini, JT ^{[1
]}

Soini, E ^{[1
]}

机构：

[1] Turku Univ, Dept Med Phys & Chem, FIN-20521 Turku, Finland

来源：

CYTOMETRY | 1999年 / 36卷 / 03期

关键词：

flow cytometry; rare-event detection; two-photon fluorescence excitation; photon burst detection; autocorrelation;

D O I：

10.1002/(SICI)1097-0320(19990701)36:3<183::AID-CYTO6>3.0.CO;2-C

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We studied the use of a dramatically reduced testing zone in combination with two-photon excitation and photon-burst analysis in high-throughput rare-event detection simulation using a modified flow cytometer. Two-photon excitation measurements were performed with a mode-locked titanium:sapphire laser. Fluorescence emission was measured with a photon-counting avalanche photodiode. Measured signal was analysed offline by autocorrelation and burst detection methods. Test samples were composed of full blood and orange fluorescent polystyrene nanospheres mixed in full blood. Results show chat two-photon fluorescence excitation and time-correlation analysis provide a good signal-to-noise ratio for rare-event particle detection in a turbid sample environment. Cytometry 36:183-188, 1999. (C) 1999 Wiley-Liss, Inc.

引用

页码：183 / 188

页数：6

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