Biofilm formation by Prototheca zopfii isolated from clinical and subclinical bovine mastitis in distinct growth conditions under different dyes

被引：4
作者：
Ely, Valessa Lunkes [1 ]
Gressler, Leticia Trevisan [2 ]
Sutili, Fernando Jonas [3 ]
Ribeiro, Marcio Garcia [4 ]
da Costa, Mateus Matiuzzi [5 ]
de Vargas, Agueda Castagna [1 ]
Botton, Sonia de Avila [1 ]
机构：
[1] Univ Fed Santa Maria, DMVP, PPGMV, PPGCF,CCR, BR-97105900 Santa Maria, RS, Brazil
[2] Inst Fed Farroupilha IFFar, Lab Microbiol & Doencas Infecciosas, Frederico Westphalen, RS, Brazil
[3] Univ Fed Santa Maria, Dept Fisiol & Farmacol, Santa Maria, RS, Brazil
[4] Univ Estadual Paulista Julio Mesquita Filho UNESP, Fac Med Vet & Zootecnia, Dept Higiene Vet & Saude Publ, Botucatu, SP, Brazil
[5] Univ Fed Vale Sao Francisco UNIVASF, Petrolina, PE, Brazil
来源：
CIENCIA RURAL | 2019年 / 49卷 / 02期
关键词：
Prototheca zopfii; dairy cattle; bovine mastitis; intramammary infections; microplate biofihn assay; virulence factor; STAPHYLOCOCCUS-AUREUS; GENOTYPES; IDENTIFICATION; SURFACES;
D O I：
10.1590/0103-8478cr20180574
中图分类号：
S3 [农学（农艺学）];
学科分类号：
0901 ;
摘要：
Prototheca spp. have been reported as an emergent environmental mastitis pathogen in several countries. Biofilm formation is a significant factor associated with different degrees of virulence developed by many microorganisms, including Prototheca spp. The present study aimed to compare two growth conditions and two staining dyes to determine which combination was more appropriate to evaluate qualitatively and quantitatively the production of biofilm by P. zopfii. Biofilm formation was evaluated in polystyrene microplates under static and dynamic growth conditions and staining with crystal violet or cotton blue dye. All P. zopfii isolates from cows with mastitis were classified as biofibn-producers in all growth conditions and staining. The cotton blue dye proved to be more appropriate method to classify the intensity of P. zopfii biofilm production.
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页数：7
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