Modification of feed intake response to a beta(2)-agonist by bovine somatotropin in lactating or dry dairy cows

The aim of this experiment was to show whether growth hormone could increase the effect of the beta(2)-adrenergic agonist clenbuterol on feed intake. Two groups of Holstein cows [lactating (n = 4) and dry (n = 4)] were used to rule out the possible effects of increased energy requirements on feed intake. Treatments were administered according to a crossover experimental design with two 13-d periods and an 8-d readjustment interval. Treatments consisted of daily injections of 40 mg of bST or a placebo. Moreover, clenbuterol (3.16 mu g/kg of BW) or saline challenges were infused intravenously for 4 h either on d 8 or on d 11 of each period. The cows were offered a dehydrated total mixed diet for ad libitum intake for 4 h twice daily. For both groups of cows, clenbuterol reduced DMI on the experimental day only, and bST intensified the effects of clenbuterol on DMI, causing a sharper decrease in DMI over 2 d. This enhancement of clenbuterol activity modified certain metabolic parameters including higher lipid mobilization during infusion and higher glycogenolysis. For dry cows, the initial rate of eating was reduced under bST treatment alone. During early lactation, bST, either by itself or through increased beta(2)-adrenergic stimulation may restrict the development of intake by dairy cows over a few weeks.