In vivo detection of nitric oxide distribution in mice

被引：13

作者：

Komarov, AM ^{[1
]}

机构：

[1] George Washington Univ, Med Ctr, Dept Physiol & Expt Med, Washington, DC 20037 USA

来源：

MOLECULAR AND CELLULAR BIOCHEMISTRY | 2002年 / 234卷 / 01期

关键词：

diethyldithiocarbamate; endotoxic shock; inducible nitric oxide synthase; in vivo EPR; nitric oxide; spin trapping;

D O I：

10.1023/A:1015911216137

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

This paper discusses in vivo detection of nitric oxide (NO) distribution in endotoxin-treated mice using L-band (1.1 GHz) electron paramagnetic resonance spectroscopy (EPR) in combination with the hydrophilic NO trapping complex: N-methyl-D-glucamine dithiocarbamate and iron (MGD-Fe). MGD-Fe-NO complex is found in the upper abdomen (liver region), lower abdomen (kidney and urinary bladder) and head region of ICR mice. Experiments with nitric oxide synthase (NOS) inhibition and N-15-labeled L-arginine as NOS substrate verify the origin of trapped NO from L-arginine. However, contribution from a 'nonenzymatic' NO generation pathway can not be ruled out. This paper further examines potential artifacts, which may arise in experiments using dithiocarbamate-iron complexes as NO trapping agents.

引用

页码：387 / 392

页数：6

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