Optimization of the synthesis of silver nanoparticles using the leaf extract of Ocimum sanctum and evaluation of their antioxidant potential

This work investigates the green synthesis of silver nanoparticles (AgNPs) utilizing the aqueous leaf extract of Ocimum sanctum L. A change in colour to dark brown from yellow and an absorption maximum at 460 nm provided evidence for the synthesis of AgNPs. Several reaction conditions namely leaf extract volume, AgNO3 concentration and duration of incubation were optimized. Maximum synthesis of AgNPs could be achieved with 1 ml of 5% aqueous leaf extract, 1 mM AgNO3 and 2.5 h incubation period. DPPH (2, 2-diphenyl - 1 - picrylhydrazyl) assay revealed that AgNPs are better than the leaf extract in terms of antioxidant potential. These results strongly recommend the application of green-synthesized AgNPs as effective antioxidants against oxidative stresses associated with degenerative diseases. Characterization of AgNPs were then carried out using certain methods namely as x-ray diffraction (XRD) measurement, Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). XRD study confirmed the synthesis of face-centered cubic-shaped AgNPs that are of crystalline nature. The mean particle size of the synthesized AgNPs computed employing the Debye-Scherrer formula, was 22 nm. FTIR study demonstrated that the AgNPs consisted of certain functional groups of O. sanctum which might have helped in reducing AgNO3 and capping AgNPs. SEM images showed the synthesis of AgNPs of various shapes viz. globular, cubical and flaky. The scattered AgNPs were found to have a size close to 20 nm, which roughly matches with the XRD analysis of the current study.