Evaluation of the environmental conditions for the continuous production of lignin peroxidase by Phanerochaete Chrysosporium in fixed-bed bioreactors

被引：9

作者：

Couto, SR ^{[1
]}

Feijoo, G ^{[1
]}

Moreira, MT ^{[1
]}

Lema, JM ^{[1
]}

机构：

[1] Univ Santiago de Compostela, Inst Technol, Dept Chem Engn, E-15706 Santiago, Spain

来源：

BIOTECHNOLOGY LETTERS | 2002年 / 24卷 / 10期

关键词：

continuous production; fixed-bed bioreactor; lignin peroxidase; Phanerochaete chrysosporium; pulsation system;

D O I：

10.1023/A:1015576121541

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A new system to produce lignin peroxidase (LiP) continuously by Phanerochaete chrysosporium is described. A fixed-bed bioreactor with a pulsing device was used as the optimal bioreactor configuration. Addition of veratryl alcohol (1 mM), tryptophan (1 mM), no Mn2+ addition, low glucose addition rate (60-70 mg l(-1) h) and an atmosphere of O-2 gave maximum LiP activities of 700 U l(-1), which are higher than those previously reported.

引用

页码：791 / 794

页数：4

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