Electrochemical Determination of Metallothioneins by the Modified Brdicka Procedure as an Analytical Tool in Biomonitoring Studies

被引：3

作者：

Mijosek, Tatjana ^{[1
]}

Erk, Marijana ^{[1
]}

Marijic, Vlatka Filipovic ^{[1
]}

Krasnici, Nesrete ^{[1
]}

Dragun, Zrinka ^{[1
]}

Ivankovic, Dusica ^{[1
]}

机构：

[1] Rudjer Boskovic Inst, Div Marine & Environm Res, Bijenicka Cesta 54, HR-10000 Zagreb, Croatia

来源：

CROATICA CHEMICA ACTA | 2018年 / 91卷 / 04期

关键词：

metallothioneins; differential pulse voltammetry; modified scan rate; fish tissues; biomarkers; biomonitoring; METALS; FISH; QUANTIFICATION; PROTEINS; LIVER;

D O I：

10.5562/cca3444

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Present study aims to improve the modified Brdicka procedure of metallothionein (MT) quantification in a way to reduce the analysis duration. Therefore, scan rate of the voltammetric sweep was increased from 0.0052 V s(-1) to 0.013 V s(-1) by lowering the duration of one step potential from 0.5 s to 0.2 s, which resulted in 2.5 times faster voltammetric sweep. Research confirmed high accuracy and reliability of MT measurements, in both standard sample and samples of heat-treated cytosols of liver, gills and the intestine of European chub and liver of brown trout. The method was confirmed as fast and reliable electrochemical technique for quantification of MTs and this time-saving improvement is especially useful and applicable in different biomonitoring studies which require the analyses of numerous biological samples and high sample throughput.

引用

页码：475 / 480

页数：6

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