Selection of DNA-binding compounds via multistage molecular evolution

被引：48

作者：

Klekota, B ^{[1
]}

Miller, BL ^{[1
]}

机构：

[1] Univ Rochester, Dept Chem, Rochester, NY 14627 USA

来源：

TETRAHEDRON | 1999年 / 55卷 / 39期

关键词：

D O I：

10.1016/S0040-4020(99)00687-0

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Combinatorial libraries incorporating multiple equilibria offer opportunities to study molecular evolution, and are a novel method of identifying ligands for biological receptors. We describe the construction and evaluation of a multi-equilibrium combinatorial library, in which structural diversity and structural mutation are accomplished via reversible imine formation and transition-metal complexation. We demonstrate that oligo d(A.T)-cellulose resin can select subsets d this library, in accord with measured solution-phase affinities. (C) 1999 Elsevier Science Ltd. All rights reserved.

引用

页码：11687 / 11697

页数：11

共 21 条

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