Impact of Oxygenation of Bioartificial Liver Using Perfluorocarbon Emulsion Perftoran on Metabolism of Human Hepatoma C3A Cells

被引：9

作者：

Kinasiewicz, Andrzej ^{[1
]}

Smietanka, Anna ^{[1
]}

Gajkowska, Barbara ^{[2
]}

Werynski, Andrzej ^{[1
]}

机构：

[1] Polish Acad Sci, Inst Biocybernet & Biomed Engn, Warsaw, Poland

[2] Polish Acad Sci, Dept Cell Ultrastruct, Med Res Ctr, Warsaw, Poland

来源：

ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY | 2008年 / 36卷 / 06期

关键词：

D O I：

10.1080/10731190802554380

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

One of the most important challenges in bioartificial liver designed for patients suffering from acute liver failure is oxygenation of cells within the bioreactor. The aim of this study was to evaluate the impact of oxygenation of bioartificial liver using perfluorocarbon (PFC) emulsion on the metabolic activity of hepatic cells in vitro. Mineral fibers coated with collagen type I were used as scaffolds for hepatic cells. Significantly higher total protein synthesis by hepatic C3A cells cultured in the bioreactor for 24 hours, in the group treated with medium supplemented with PFC emulsion, was observed in comparison with medium without PFC. Albumin production increased in the group treated with PFC after 1 hour of perfusion and was continuously, statistically, significantly higher during perfusion then the control group. In conclusion, the use of oxygen carriers, such as the PFC emulsion, can significantly improve synthetic performance of the bioreactor. Mineral fibers coated with extracellular proteins may serve as support for hepatic cells in the bioreactor.

引用

页码：525 / 534

页数：10

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