CRISPR-Cas9: A Revolutionary Tool for Cancer Modelling

被引：26

作者：

Torres-Ruiz, Raul ^{[1
]}

Rodriguez-Perales, Sandra ^{[2
]}

机构：

[1] Fdn Ctr Nacl Invest Cardiovasc CNIC, Viral Vector Tech Unit, Madrid 28029, Spain

[2] Ctr Nacl Invest Oncol, Human Canc Genet Program, Mol Cytogenet Grp, Madrid 28029, Spain

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2015年 / 16卷 / 09期

关键词：

CRISPR-Cas9; system; cancer modelling; gene mutations; rearrangements; genome engineering; OFF-TARGET MUTATIONS; ONE-STEP GENERATION; CHROMOSOMAL REARRANGEMENTS; GENE DELIVERY; MOUSE MODELS; HUMAN-CELLS; GENOME; RNA; CAS9; CRISPR/CAS9;

D O I：

10.3390/ijms160922151

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The cancer-modelling field is now experiencing a conversion with the recent emergence of the RNA-programmable CRISPR-Cas9 system, a flexible methodology to produce essentially any desired modification in the genome. Cancer is a multistep process that involves many genetic mutations and other genome rearrangements. Despite their importance, it is difficult to recapitulate the degree of genetic complexity found in patient tumors. The CRISPR-Cas9 system for genome editing has been proven as a robust technology that makes it possible to generate cellular and animal models that recapitulate those cooperative alterations rapidly and at low cost. In this review, we will discuss the innovative applications of the CRISPR-Cas9 system to generate new models, providing a new way to interrogate the development and progression of cancers.

引用

页码：22151 / 22168

页数：18

共 78 条

[1] Cancer-Associated Protein Kinase C Mutations Reveal Kinase's Role as Tumor Suppressor [J].

Antal, Corina E. ;

Hudson, Andrew M. ;

Kang, Emily ;

Zanca, Ciro ;

Wirth, Christopher ;

Stephenson, Natalie L. ;

Trotter, Eleanor W. ;

Gallegos, Lisa L. ;

Miller, Crispin J. ;

Fumari, Frank B. ;

Hunter, Tony ;

Brognard, John ;

Newton, Alexandra C. .

CELL, 2015, 160 (03) :489-502

[2] A prudent path forward for genomic engineering and germline gene modification [J].

Baltimore, David ;

Berg, Paul ;

Botchan, Michael ;

Carroll, Dana ;

Charo, R. Alta ;

Church, George ;

Corn, Jacob E. ;

Daley, George Q. ;

Doudna, Jennifer A. ;

Fenner, Marsha ;

Greely, Henry T. ;

Jinek, Martin ;

Martin, G. Steven ;

Penhoet, Edward ;

Puck, Jennifer ;

Sternberg, Samuel H. ;

Weissman, Jonathan S. ;

Yamamoto, Keith R. .

SCIENCE, 2015, 348 (6230) :36-38

[3] Modes of resistance to anti-angiogenic therapy [J].

Bergers, Gabriele ;

Hanahan, Douglas .

NATURE REVIEWS CANCER, 2008, 8 (08) :592-603

[4] Simple and Rapid In Vivo Generation of Chromosomal Rearrangements using CRISPR/Cas9 Technology [J].

Blasco, Rafael B. ;

Karaca, Elif ;

Ambrogio, Chiara ;

Cheong, Taek-Chin ;

Karayol, Emre ;

Minero, Valerio G. ;

Voena, Claudia ;

Chiarle, Roberto .

CELL REPORTS, 2014, 9 (04) :1219-1227

[5] Gene targeting in mice: functional analysis of the mammalian genome for the twenty-first century [J].

Capecchi, MR .

NATURE REVIEWS GENETICS, 2005, 6 (06) :507-512

[6] ALTERING THE GENOME BY HOMOLOGOUS RECOMBINATION [J].

CAPECCHI, MR .

SCIENCE, 1989, 244 (4910) :1288-1292

[7]

Carter PJ, 2000, INT J MOL MED, V6, P17

[8] Cripto-1 as a novel therapeutic target for triple negative breast cancer [J].

Castro, Nadia P. ;

Fedorova-Abrams, Natalie D. ;

Merchant, Anand S. ;

Rangel, Maria Cristina ;

Nagaoka, Tadahiro ;

Karasawa, Hideaki ;

Klauzinska, Malgorzata ;

Hewitt, Stephen M. ;

Biswas, Kajal ;

Sharan, Shyam K. ;

Salomon, David S. .

ONCOTARGET, 2015, 6 (14) :11910-11929

[9] MLL3 Is a Haploinsufficient 7q Tumor Suppressor in Acute Myeloid Leukemia [J].

Chen, Chong ;

Liu, Yu ;

Rappaport, Amy R. ;

Kitzing, Thomas ;

Schultz, Nikolaus ;

Zhao, Zhen ;

Shroff, Aditya S. ;

Dickins, Ross A. ;

Vakoc, Christopher R. ;

Bradner, James E. ;

Stock, Wendy ;

LeBeau, Michelle M. ;

Shannon, Kevin M. ;

Kogan, Scott ;

Zuber, Johannes ;

Lowe, Scott W. .

CANCER CELL, 2014, 25 (05) :652-665

[10] Genome-wide CRISPR Screen in a Mouse Model of Tumor Growth and Metastasis [J].

Chen, Sidi ;

Sanjana, Neville E. ;

Zheng, Kaijie ;

Shalem, Ophir ;

Lee, Kyungheon ;

Shi, Xi ;

Scott, David A. ;

Song, Jun ;

Pan, Jen Q. ;

Weissleder, Ralph ;

Lee, Hakho ;

Zhang, Feng ;

Sharp, Phillip A. .

CELL, 2015, 160 (06) :1246-1260

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