Chemical synthesis of oligonucleotides containing the (6-4) photoproduct at the thymine-cytosine site and its repair by (6-4) photolyase

被引：1
|
作者：
Iwai, S
Mizukoshi, T
Hitomi, K
Todo, T
机构：
[1] Biomol Engn Res Inst, Suita, Osaka 5650874, Japan
[2] Kyoto Univ, Ctr Radiat Biol, Sakyo Ku, Kyoto 6068501, Japan
来源：
NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS | 1999年 / 18卷 / 6-7期
关键词：
D O I：
10.1080/07328319908044706
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A phosphoramidite building block of the T(6-4)C photoproduct was synthesized. One of the differences from T(6-4)T was formation of cytosine hydrates by UV irradiation, and the other was acylation of the amino function with the capping reagent. The capping step was omitted to improve the yield of the desired oligonucleotides. Characterization of the (6-4) photolyase using one of the oligonucleotides revealed that this enzyme restores the pyrimidines in T(6-4)C to their original structures.
引用
收藏
页码：1325 / 1327
页数：3
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