Polyclonal antibody to porcine p53 protein: A new tool for studying the p53 pathway in a porcine model

被引：22

作者：

Qiu, Yafeng ^{[1
]}

Shen, Yang ^{[1
]}

Li, Xiangdong ^{[1
]}

Liu, Qingwei ^{[1
]}

Ma, Zhiyong ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, Shanghai Vet Res Inst, Shanghai 200241, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2008年 / 377卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Anti-porcine p53 antibody; Porcine p53; Polyclonal antibody; Signaling pathway;

D O I：

10.1016/j.bbrc.2008.09.117

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Although the tumor Suppressor protein p53 is important in the control of various cellular activities, the analysis of p53 in the porcine model has been hampered by a lack of a suitable antibody that is specific for porcine p53. Using a recombinant porcine p53, we generated a rabbit polyclonal antibody (designated SH0797) that is directed against porcine p53. The results of the study show that the antibody is capable of detecting recombinant p53 protein expressed in Escherichia coli, as well as FLAG-tagged p53 that is expressed ill ill, transfected cells, This demonstrates that the antibody is specific for the porcine p53 protein. The antibody also showed the ability to immunoprecipitate p53 protein from extracts of porcine cells and to cross-react with human p53 protein. In addition, expression of porcine p53 could be induced readily in porcine cells and detected using this new tool. I-his antibody is a useful tool for Use in studies of the cellular pathways that involve p53 in the porcine model. (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：151 / 155

页数：5

共 20 条

[1] Savior and slayer: the two faces of p53 [J].

Bensaad, K ;

Vousden, KH .

NATURE MEDICINE, 2005, 11 (12) :1278-1279

[2] TIGAR, a p53-inducible regulator of glycolysis and apoptosis [J].

Bensaad, Karim ;

Tsuruta, Atsushi ;

Selak, Mary A. ;

Calvo Vidal, M. Nieves ;

Nakano, Katsunori ;

Bartrons, Ramon ;

Gottlieb, Eyal ;

Vousden, Karen H. .

CELL, 2006, 126 (01) :107-120

[3] Nucleotide sequence of the porcine p53 cDNA, and the detection of recombinant porcine p53 expressed in vitro with a variety of anti-p53 antibodies [J].

Burr, PD ;

Argyle, DJ ;

Reid, SWJ ;

Nasir, L .

ONCOGENE, 1999, 18 (35) :5005-5009

[4] DRAM, a p53-induced modulator of autophagy, is critical for apoptosis [J].

Crighton, Diane ;

Wilkinson, Simon ;

O'Prey, Jim ;

Syed, Nelofer ;

Smith, Paul ;

Harrison, Paul R. ;

Gasco, Milena ;

Garrone, Ornella ;

Crook, Tim ;

Ryan, Kevin M. .

CELL, 2006, 126 (01) :121-134

[5]

FRIEDMAN L, 1995, J ANIM SCI, V73, P1340

[6] Lipopolysaccharide prevents doxorubicin-induced apoptosis in RAW 264.7 macrophage cells by inhibiting p53 activation [J].

Hassan, F ;

Islam, S ;

Mu, MM ;

Ito, H ;

Koide, N ;

Mori, I ;

Yoshida, T ;

Yokochi, T .

MOLECULAR CANCER RESEARCH, 2005, 3 (07) :373-379

[7] p53: 25 years after its discovery [J].

Hofseth, LJ ;

Hussain, SP ;

Harris, CC .

TRENDS IN PHARMACOLOGICAL SCIENCES, 2004, 25 (04) :177-181

[8] Transcriptional regulation by p53: one protein, many possibilities [J].

Laptenko, O. ;

Prives, C. .

CELL DEATH AND DIFFERENTIATION, 2006, 13 (06) :951-961

[9] p53 regulates mitochondrial respiration [J].

Matoba, Satoaki ;

Kang, Ju-Gyeong ;

Patino, Willmar D. ;

Wragg, Andrew ;

Boehm, Manfred ;

Gavrilova, Oksana ;

Hurley, Paula J. ;

Bunz, Fred ;

Hwang, Paul M. .

SCIENCE, 2006, 312 (5780) :1650-1653

[10] THE PIG AS A MODEL FOR HUMAN-NUTRITION [J].

MILLER, ER ;

ULLREY, DE .

ANNUAL REVIEW OF NUTRITION, 1987, 7 :361-382

← 1 2 →