Understanding the Nonproductive Enzyme Adsorption and Physicochemical Properties of Residual Lignins in Moso Bamboo Pretreated with Sulfuric Acid and Kraft Pulping

被引:60
作者
Huang, Caoxing [1 ,2 ]
He, Juan [1 ,2 ]
Min, Douyong [3 ]
Lai, Chenhuan [1 ,2 ]
Yong, Qiang [1 ,2 ]
机构
[1] Nanjing Forestry Univ, Coinnovat Ctr Efficient Proc & Utilizat Forest Pr, Nanjing 210037, Jiangsu, Peoples R China
[2] Nanjing Forestry Univ, Coll Chem Engn, Nanjing 210037, Jiangsu, Peoples R China
[3] Nanjing Forestry Univ, Coll Light Ind Sci & Engn, Nanjing 210037, Jiangsu, Peoples R China
基金
中国国家自然科学基金;
关键词
Moso bamboo; Pretreatment; Nonproductive adsorption; Adsorption isotherm; Physicochemical properties; SODIUM-HYDROXIDE; AQUEOUS AMMONIA; HYDROLYSIS; CELLULASE; SACCHARIFICATION; DIGESTIBILITY; AUTOHYDROLYSIS; INHIBITION; FRACTIONS; SOFTWOOD;
D O I
10.1007/s12010-016-2183-8
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In this work, to elucidate why the acid-pretreated bamboo shows disappointingly low enzymatic digestibility comparing to the alkali-pretreated bamboo, residual lignins in acid-pretreated and kraft pulped bamboo were isolated and analyzed by adsorption isotherm to evaluate their extents of nonproductive enzyme adsorption. Meanwhile, physicochemical properties of the isolated lignins were analyzed and a relationship was established with non-productive adsorption. Results showed that the adsorption affinity and binding strength of cellulase on acid-pretreated bamboo lignin (MWLa) was significantly higher than that on residual lignin in pulped bamboo (MWLp). The maximum adsorption capacity of cellulase on MWLp was 129.49 mg/g lignin, which was lower than that on MWLa (160.25 mg/g lignin). When isolated lignins were added into the Avicel hydrolysis solution, the inhibitory effect on enzymatic hydrolysis efficiency of MWLa was found to be considerably stronger than that with MWLp. The cellulase adsorption on isolated lignins was correlated positively with hydrophobicity, phenolic hydroxyl group, and degree of condensation but negatively with surface charges and aliphatic hydroxyl group. These results suggest that the higher nonproductive cellulase adsorption and physicochemical properties of residual lignin in acid-pretreated bamboo may be responsible for its disappointingly low enzymatic digestibility.
引用
收藏
页码:1508 / 1523
页数:16
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