The Effect of the Microgravity Rotating Culture System on the Chondrogenic Differentiation of Bone Marrow Mesenchymal Stem Cells

被引：40
|
作者：
Wu, Xing [1 ]
Li, Shao-hua [1 ]
Lou, Lie-ming [1 ]
Chen, Zheng-rong [2 ]
机构：
[1] Tongji Univ, Sch Med, Shanghai Peoples Hosp 10, Dept Orthopaed, Shanghai 200072, Peoples R China
[2] Fudan Univ, Sch Med, Shanghai Zhongshan Hosp, Dept Orthopaed, Shanghai 200032, Peoples R China
来源：
MOLECULAR BIOTECHNOLOGY | 2013年 / 54卷 / 02期
关键词：
Microgravity rotating culture system; Marrow mesenchymal stem cells (MSCs); Chondrogenic differentiation; IN-VITRO CHONDROGENESIS; PROGENITOR CELLS; CARTILAGE; CULTIVATION; SCAFFOLDS;
D O I：
10.1007/s12033-012-9568-x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We investigated the influence of the microgravity rotating culture system on the chondrogenic differentiation of bone marrow mesenchymal stem cells (MSCs). During chondrogenic induction, MSCs combined with polyglycolic acid (PGA) were cultured by static culture or microgravity rotating culture and chondrocyte formation was confirmed by toluidine blue staining. Furthermore, the mRNA and protein expressions of a specific cartilage extracellular matrix protein (collagen type II and Aggrecan) were evaluated by real-time RT-PCR and western blot, respectively. Toluidine blue staining indicated the OD values of proteoglycans semi-determination were higher in the microgravity rotating culture group than the static culture group. Following chondrogenic induction, mRNA and proteins of collagen type II and Aggrecan were more significantly expressed in cells of the microgravity rotating culture group compared with the controls. Compared with routine three-dimensional static culture, the microgravity rotating culture system was more effective for the construction of tissue-engineered cartilage in vitro.
引用
收藏
页码：331 / 336
页数：6
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