Cloning and characterization of the crystal protein-encoding gene of Bacillus thuringiensis subsp yunnanensis

被引：9

作者：

Balasubramanian, P ^{[1
]}

Jayakumar, R ^{[1
]}

Shambharkar, P ^{[1
]}

Unnamalai, N ^{[1
]}

Pandian, SK ^{[1
]}

Kumaraswami, NS ^{[1
]}

Ilangovan, R ^{[1
]}

Sekar, V ^{[1
]}

机构：

[1] Madurai Kamaraj Univ, Sch Biotechnol, Dept Mol Microbiol, Madurai 625021, Tamil Nadu, India

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2002年 / 68卷 / 01期

关键词：

D O I：

10.1128/AEM.68.1.408-411.2002

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Molecular cloning and characterization of a novel cry gene, cry32Aa, of Bacillus thuringiensis subsp. yunnanensis was carried out. The Cry32Aa protein was predicted to have a molecular mass of 139.2 kDa and was found to have an unusual 42-amino-acid-long tail at the C terminus. The cry32Aa gene was localized on the 103-MDa plasmid of the organism. Bioassays showed no toxicity against several moths and mosquitoes. However, it exhibited weak toxicity against larvae of the diamondback moth, Plutella xylostella.

引用

页码：408 / 411

页数：4

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