Surface plasmon resonance: a label-free tool for cellular analysis

被引:29
|
作者
Zeidan, Effat [1 ]
Kepley, Christopher L. [1 ]
Sayes, Christie [1 ]
Sandros, Marinella G. [1 ]
机构
[1] Univ N Carolina, Dept Nanosci, Greensboro, NC 27401 USA
关键词
bacterial adherence properties; biomolecular interactions; cell sorting; cellular detection; cellular morphology alterations; cellular secretions; long-range surface plasmon resonance; nanoscale cellular dynamics; optical biosensor; single-cell analysis; IMAGING MEASUREMENTS; QUANTUM DOTS; LIVING CELLS; STEM-CELLS; SPR; ADHESION; SENSITIVITY; BIOSENSOR; BIOFILMS; BINDING;
D O I
10.2217/nnm.15.31
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Surface plasmon resonance (SPR) is a popular technique that allows for sensitive, specific, label-free and real-time assessment of biomolecular interactions. SPR is a nondestructive, modular and flexible tool for various applications in biomedical sciences ranging from cell sorting, cell surface characterization and drug discovery. In this review, we will discuss more specifically how SPR is used to monitor the dynamics of various types of cellular binding events and morphological adherence changes in response to external stimuli.
引用
收藏
页码:1833 / 1846
页数:14
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