cerebral ischemia;
DNA methylation;
DNA methyltransferase;
epigenetics;
stroke;
D O I:
10.1097/00001756-200112040-00032
中图分类号:
Q189 [神经科学];
学科分类号:
071006 ;
摘要:
DNA methylation is important for controlling gene expression and is catalyzed by DNA methyltransferase (DnmtI) an enzyme abundant in brain. We recently demonstrated that, mice expressing reduced levels of DnmtI are protected from cerebral ischemia. Here, we used the cre/IoxP system to produce conditional mutants that lack Dnmt I in postmitotic neurons of the postnatal brain. We demonstrate that animals heterozygous for the conditional allele (DnmtI(llox/+)) have significantly smaller infarcts following Ih middle cerebral artery occlusion/reperfusion compared to their wildtype litters. Surprisingly, mice with a deletion of DnmtI in post-mitotic neurons (DnmtI(llox/c)) were not protected. In conclusion, we demonstrate that reduced levels of DnmtI, but not its absence, in post-mitotic neurons protect from ischemic brain injury. NeuroReport 12:3763-3766 (C) 2001 Lippincott Williams & Wilkins.
机构:
UNIV SO CALIF, SCH MED,DEPT BIOCHEM & MOL BIOL,UROL CANC RES LAB, NORRIS COMPREHENS CANC CTR, LOS ANGELES, CA 90033 USAUNIV SO CALIF, SCH MED,DEPT BIOCHEM & MOL BIOL,UROL CANC RES LAB, NORRIS COMPREHENS CANC CTR, LOS ANGELES, CA 90033 USA
Gonzalgo, ML
;
Jones, PA
论文数: 0引用数: 0
h-index: 0
机构:
UNIV SO CALIF, SCH MED,DEPT BIOCHEM & MOL BIOL,UROL CANC RES LAB, NORRIS COMPREHENS CANC CTR, LOS ANGELES, CA 90033 USAUNIV SO CALIF, SCH MED,DEPT BIOCHEM & MOL BIOL,UROL CANC RES LAB, NORRIS COMPREHENS CANC CTR, LOS ANGELES, CA 90033 USA
机构:
UNIV SO CALIF, SCH MED,DEPT BIOCHEM & MOL BIOL,UROL CANC RES LAB, NORRIS COMPREHENS CANC CTR, LOS ANGELES, CA 90033 USAUNIV SO CALIF, SCH MED,DEPT BIOCHEM & MOL BIOL,UROL CANC RES LAB, NORRIS COMPREHENS CANC CTR, LOS ANGELES, CA 90033 USA
Gonzalgo, ML
;
Jones, PA
论文数: 0引用数: 0
h-index: 0
机构:
UNIV SO CALIF, SCH MED,DEPT BIOCHEM & MOL BIOL,UROL CANC RES LAB, NORRIS COMPREHENS CANC CTR, LOS ANGELES, CA 90033 USAUNIV SO CALIF, SCH MED,DEPT BIOCHEM & MOL BIOL,UROL CANC RES LAB, NORRIS COMPREHENS CANC CTR, LOS ANGELES, CA 90033 USA