Enhancing the production of eicosapentaenoic acid (EPA) from Nannochloropsis oceanica CY2 using innovative photobioreactors with optimal light source arrangements

被引：49
作者：
Chen, Chun-Yen [1 ]
Chen, Yu-Chun [2 ]
Huang, Hsiao-Chen [2 ]
Ho, Shih-Hsin [3 ]
Chang, Jo-Shu [2 ,4 ]
机构：
[1] Natl Cheng Kung Univ, Ctr Biosci & Biotechnol, Tainan 701, Taiwan
[2] Natl Cheng Kung Univ, Dept Chem Engn, Tainan 701, Taiwan
[3] Kobe Univ, Org Adv Sci & Technol, Kobe, Hyogo 657, Japan
[4] Natl Cheng Kung Univ, Res Ctr Energy Technol & Strategy, Tainan 701, Taiwan
来源：
BIORESOURCE TECHNOLOGY | 2015年 / 191卷
基金：
日本科学技术振兴机构;
关键词：
EPA; Nannochloropsis oceanica; Immersed light source; LED; Flashing frequency; LIPID PRODUCTION; MARINE MICROALGAE; CELL-GROWTH; FATTY-ACIDS; ACCUMULATION; CULTIVATION; NITROGEN; STRATEGIES; INTENSITY; OCULATA;
D O I：
10.1016/j.biortech.2015.03.001
中图分类号：
S2 [农业工程];
学科分类号：
0828 ;
摘要：
Binary combinations of LEDs with four different colors were used as light sources to identify the effects of multiple wavelengths on the production of eicosapentaenoic acid (EPA) by an isolated microalga Nannochloropsis oceanica CY2. Combining LED-Blue and LED-Red could give the highest EPA productivity of 13.24 mg L-1 d(-1), which was further enhanced to 14.4 mg L-1 d(-1) when using semi-batch operations at a 40% medium replacement ratio. A novel photobioreactor with additional immersed light sources improved light penetration efficiency and led to an 38% (0.170-0.235 g L-1 d(-1)) increase in the microalgae biomass productivity and a 9% decrease in electricity consumption yield of EPA (10.15-9.33 kW-h (g EPA)(-1)) when compared with the control (i.e., without immersed light sources). Operating the immersed LEDs at a flashing-frequency of 9 Hz further lowered the energy consumption yield to 8.87 kW-h (g EPA)(-1). (C) 2015 Elsevier Ltd. All rights reserved.
引用
收藏
页码：407 / 413
页数：7
相关论文
共 28 条
[1]   Microalgal biofactories: a promising approach towards sustainable omega-3 fatty acid production [J].
Adarme-Vega, T. Catalina ;
Lim, David K. Y. ;
Timmins, Matthew ;
Vernen, Felicitas ;
Li, Yan ;
Schenk, Peer M. .
MICROBIAL CELL FACTORIES, 2012, 11
[2]   Polyunsaturated fatty acids in the central nervous system:: evolution of concepts and nutritional implications throughout life [J].
Alessandri, JM ;
Guesnet, P ;
Vancassel, S ;
Astorg, P ;
Denis, I ;
Langelier, B ;
Aïd, S ;
Poumès-Ballihaut, C ;
Champeil-Potokar, G ;
Lavialle, M .
REPRODUCTION NUTRITION DEVELOPMENT, 2004, 44 (06) :509-538
[3]   Dynamic angling effort influences the value of minimum-length limits to prevent recruitment overfishing [J].
Allen, M. S. ;
Ahrens, R. N. M. ;
Hansen, M. J. ;
Arlinghaus, R. .
FISHERIES MANAGEMENT AND ECOLOGY, 2013, 20 (2-3) :247-257
[4]   Engineering strategies for enhancing the production of eicosapentaenoic acid (EPA) from an isolated microalga Nannochloropsis oceanica CY2 [J].
Chen, Chun-Yen ;
Chen, Yu-Chun ;
Huang, Hsiao-Chen ;
Huang, Chieh-Chen ;
Lee, Wen-Lung ;
Chang, Jo-Shu .
BIORESOURCE TECHNOLOGY, 2013, 147 :160-167
[5]   Lipid accumulation and CO2 utilization of Nannochloropsis oculata in response to CO2 aeration [J].
Chiu, Sheng-Yi ;
Kao, Chien-Ya ;
Tsai, Ming-Ta ;
Ong, Seow-Chin ;
Chen, Chiun-Hsun ;
Lin, Chih-Sheng .
BIORESOURCE TECHNOLOGY, 2009, 100 (02) :833-838
[6]  
Connor WE, 2000, AM J CLIN NUTR, V71, p171S, DOI 10.1093/ajcn/71.1.171S
[7]   Enhanced algae growth in both phototrophic and mixotrophic culture under blue light [J].
Das, Probir ;
Lei, Wang ;
Aziz, Siti Sarah ;
Obbard, Jeffrey Philip .
BIORESOURCE TECHNOLOGY, 2011, 102 (04) :3883-3887
[8]   Changes in the cell composition of the marine microalga, Nannochloropsis gaditana, during a light:dark cycle [J].
Fábregas, J ;
Maseda, A ;
Domínguez, A ;
Ferreira, M ;
Otero, A .
BIOTECHNOLOGY LETTERS, 2002, 24 (20) :1699-1703
[9]   Effect of UV stress on the fatty acid and lipid class composition in two marine microalgae Pavlova lutheri (Pavlovophyceae) and Odontella aurita (Bacillariophyceae) [J].
Guiheneuf, Freddy ;
Fouqueray, Manuela ;
Mimouni, Virginie ;
Ulmann, Lionel ;
Jacquette, Boris ;
Tremblin, Gerard .
JOURNAL OF APPLIED PHYCOLOGY, 2010, 22 (05) :629-638
[10]   Enhancing lutein productivity of an indigenous microalga Scenedesmus obliquus FSP-3 using light-related strategies [J].
Ho, Shih-Hsin ;
Chan, Ming-Chang ;
Liu, Chen-Chun ;
Chen, Chun-Yen ;
Lee, Wen-Lung ;
Lee, Duu-Jong ;
Chang, Jo-Shu .
BIORESOURCE TECHNOLOGY, 2014, 152 :275-282
123