Acacetin inhibits expression of matrix metalloproteinases via a MAPK-dependent mechanism in fibroblast-like synoviocytes

被引：23

作者：

Chen, Wei-Ping ^{[1
]}

Yang, Zhi-Gao ^{[1
]}

Hu, Peng-Fei ^{[1
]}

Bao, Jia-Peng ^{[1
]}

Wu, Li-Dong ^{[1
]}

机构：

[1] Zhejiang Univ, Sch Med, Affiliated Hosp 2, Dept Orthoped Surg, Hangzhou 310003, Zhejiang, Peoples R China

来源：

JOURNAL OF CELLULAR AND MOLECULAR MEDICINE | 2015年 / 19卷 / 08期

基金：

中国国家自然科学基金;

关键词：

acacetin; rheumatoid arthritis; matrix metalloproteinase; fibroblast-like synoviocytes; interleukin-1; NF-KAPPA-B; RHEUMATOID-ARTHRITIS; INFLAMMATION; CYTOKINES; CELLS; ACTIVATION; DISEASES; JOINT;

D O I：

10.1111/jcmm.12564

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

It is well known that rheumatoid arthritis (RA) is an autoimmune joint disease in which fibroblast-like synoviocytes (FLSs) play a pivotal role. In this study, we investigated the anti-arthritic properties of acacetin in FLSs. The expression of matrix metalloproteinase (MMP)-1, MMP-3 and MMP-13 were investigated by quantitative RT-PCR and western blot at gene and protein levels. At the same time, the phosphorylation of mitogen-activated protein kinases (MAPK) was investigated. The DNA-binding activity of NF-B was investigated by electrophoretic mobility shift assay. We found that acacetin inhibits p38 and JNK phosphorylation and reduces MMP-1, MMP-3 and MMP-13 expression in interleukin-1-induced FLSs. Our results suggest that acacetin has antiarthritic effects in FLSs. Thus, acacetin should be further studied for the treatment of arthritis.

引用

页码：1910 / 1915

页数：6

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