Altererythrobacter aerophilus sp. nov., isolated from deep-sea water of the north-west Pacific

A Gram-stain-negative, rod-shaped bacterium, designated Ery1(T), was isolated from deep-sea seawater collected from the Mariana Trench and subjected to a polyphasic investigation for taxonomy. Strain Ery1(T) was able to grow in medium containing 0-10 % NaCl (w/v; optimum, 0-1.0 %), pH 5.0-9.5 (optimum, pH 6.0-7.0) and at temperatures between 10-45 degrees C (optimum, 30-40 degrees C). The comparison of 16S rRNA gene sequences revealed that strain Ery1(T) showed highest similarity to Altererythrobacter xinjiangensis S3-63(T) (97.7 %) and Altererythrobacter rigui WW3(T) (97.6 %), and exhibited less than 97.5 % sequence similarity to other type strains of the species with validly published names. Phylogenetic analyses indicated that strain Ery1(T) fell within the cluster comprising the Altererythrobacter species and formed a coherent clade with Altererythrobacter xinjiangensis and Altererythrobacter soli. The OrthoANlu and in silico DNA-DNA hybridization values between strain Ery1(T) and the reference strains were 73.8-75.9 %and 19.2-20.1%, respectively. Strain Ery1(T) contained Q-10 as the major respiratory quinone and Q-11 in a minor amount. The major fatty acids (>10%) were summed feature 8 (C-18 : 1 omega 7c and/or C-18 (: )1 omega 6c), summed feature 3 (C-16 : (1)omega 7c and/or C-16 : 1 omega 6C), C-1(6 ):( 0), C-18 : 1 omega 7C 11-methyl and C-1(4 ):( 0) 2-OH. The major polar lipids were sphingoglycolipid, diphosphatidylglycerol, phosphatidyglycerol, phatidylethanolamine, phosphatidylcholine and three unidentified glycolipids. Differential phenotypic properties, chemotaxonomic differences, phylogenetic distinctiveness, together with the genomic data demonstrated that strain Ery1(T) represents a novel species of the genus Altererythrobacter, for which named as Altererythrobacter aerophilus sp. nov. with the type strain Ery1(T) (=KCTC 62387(T) =CGMCC 1.16499(T) =MCCC 1A10037(T)).