Multicenter validation of the VITEK MS v2.0 MALDI-TOF mass spectrometry system for the identification of fastidious gram-negative bacteria

被引:27
|
作者
Branda, John A. [1 ,2 ]
Rychert, Jenna [1 ,2 ]
Burnham, Carey-Ann D. [4 ]
Bythrow, Maureen [5 ]
Garner, Omai B.
Ginocchio, Christine C. [5 ,6 ]
Jennemann, Rebecca [3 ]
Lewinski, Michael A. [7 ]
Manji, Ryhana [5 ]
Mochon, A. Brian [7 ]
Procop, Gary W. [8 ]
Richter, Sandra S. [8 ]
Sercia, Linda F. [8 ]
Westblade, Lars F. [3 ,4 ,6 ]
Ferraro, Mary Jane [1 ,2 ]
机构
[1] Massachusetts Gen Hosp, Dept Pathol, Boston, MA 02114 USA
[2] Harvard Univ, Sch Med, Boston, MA USA
[3] Barnes Jewish Hosp, St Louis, MO 63110 USA
[4] Washington Univ, Sch Med, Dept Pathol & Immunol, St Louis, MO USA
[5] Dept Pathol & Lab Med, North Shore LIJ Hlth Syst Labs, Lake Success, NY USA
[6] Hofstra North Shore LIJ Sch Med, Dept Pathol & Lab Med, Hempstead, NY USA
[7] Univ Calif Los Angeles, David Geffen Sch Med, Dept Pathol & Lab Med, Los Angeles, CA 90095 USA
[8] Cleveland Clin, Dept Clin Pathol, Cleveland, OH 44106 USA
关键词
MALDI-TOF MS; Fastidious; Gram-negative bacilli; Identification; VITEK; DESORPTION IONIZATION-TIME; NEISSERIA; HAEMOPHILUS;
D O I
10.1016/j.diagmicrobio.2013.08.013
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
The VITEK MS v2.0 MALDI-TOF mass spectrometry system's performance in identifying fastidious gram-negative bacteria was evaluated in a multicenter study. Compared with the reference method (DNA sequencing), the VITEK MS system provided an accurate, species-level identification for 96% of 226 isolates; an additional 1% were accurately identified to the genus level. (C) 2014 Elsevier Inc. All rights reserved.
引用
收藏
页码:129 / 131
页数:3
相关论文
共 50 条
  • [11] Evaluation of MALDI-TOF mass spectrometry for the identification of bacteria growing as biofilms
    Gaudreau, A. M.
    Labrie, J.
    Goetz, C.
    Dufour, S.
    Jacques, M.
    JOURNAL OF MICROBIOLOGICAL METHODS, 2018, 145 : 79 - 81
  • [12] Identification of Gram-positive anaerobic cocci by MALDI-TOF mass spectrometry
    Veloo, A. C. M.
    Erhard, M.
    Welker, M.
    Welling, G. W.
    Degener, J. E.
    SYSTEMATIC AND APPLIED MICROBIOLOGY, 2011, 34 (01) : 58 - 62
  • [13] Evaluation of MALDI-TOF mass spectrometry for identification of anaerobic bacteria
    Barba, M. J.
    Fernandez, A.
    Oviano, M.
    Fernandez, B.
    Velasco, D.
    Bou, G.
    ANAEROBE, 2014, 30 : 126 - 128
  • [14] Rapid identification of Gram-negative organisms from blood culture bottles using a modified extraction method and MALDI-TOF mass spectrometry
    Gray, Timothy J.
    Thomas, Lee
    Olma, Tom
    Iredell, Jonathan R.
    Chen, Sharon C. -A.
    DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2013, 77 (02) : 110 - 112
  • [15] An Automated Sample Preparation Instrument to Accelerate Positive Blood Cultures Microbial Identification by MALDI-TOF Mass Spectrometry (Vitek®MS)
    Broyer, Patrick
    Perrot, Nadine
    Rostaing, Herve
    Blaze, Jerome
    Pinston, Frederic
    Gervasi, Gaspard
    Charles, Marie-Helene
    Dachaud, Fabien
    Dachaud, Jacques
    Moulin, Frederic
    Cordier, Sylvain
    Dauwalder, Olivier
    Meugnier, Helene
    Vandenesch, Francois
    FRONTIERS IN MICROBIOLOGY, 2018, 9
  • [16] Comparing the VITEK 2 ANC card, species-specific PCR, and MALDI-TOF mass spectrometry methods for identification of lactic acid bacteria
    Kim, Sung-Youn
    Park, Sol Yi
    Jin, Ji-Eun
    Hong, Kyong-Suk
    Kim, Dae-Jung
    Kim, Yong-Kyoung
    Shin, Hee-Chang
    Cho, Soon-Kil
    Kang, Dong-Hyun
    JOURNAL OF FOOD SCIENCE, 2022, 87 (11) : 5099 - 5106
  • [17] Optimization of MALDI-ToF mass spectrometry for yeast identification: a multicenter study
    Normand, Anne-Cecile
    Gabriel, Frederic
    Riat, Arnaud
    Cassagne, Carole
    Bourgeois, Nathalie
    Huguenin, Antoine
    Chauvin, Pamela
    De Geyter, Deborah
    Bexkens, Michiel
    Rubio, Elisa
    Hendrickx, Marijke
    Ranque, Stephane
    Piarroux, Renaud
    MEDICAL MYCOLOGY, 2020, 58 (05) : 639 - 649
  • [18] Advancement in the routine identification of anaerobic bacteria by MALDI-TOF mass spectrometry
    Coltella, L.
    Mancinelli, L.
    Onori, M.
    Lucignano, B.
    Menichella, D.
    Sorge, R.
    Raponi, M.
    Mancini, R.
    Russo, C.
    EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 2013, 32 (09) : 1183 - 1192
  • [19] Use of MALDI-TOF mass spectrometry for identification of bacteria that are difficult to culture
    Biswas, Silpak
    Rolain, Jean-Marc
    JOURNAL OF MICROBIOLOGICAL METHODS, 2013, 92 (01) : 14 - 24
  • [20] Evaluation of filter paper as a means of transporting inactivated Gram-negative non-fermentative bacteria and Haemophilus spp. for identification using the MALDI-TOF MS system
    Carneiro, M. S.
    Crispim, M. N.
    Wilhelm, C. M.
    Volpato, F. C. Z.
    Barth, A. L.
    LETTERS IN APPLIED MICROBIOLOGY, 2022, 75 (01) : 17 - 23