MicroRNA expression profile and functional analysis reveal that miR-382 is a critical novel gene of alcohol addiction

被引：58

作者：

Li, Jingyuan ^{[1
]}

Li, Jing ^{[2
]}

Liu, Xiaojun ^{[1
]}

Qin, Shanshan ^{[1
]}

Guan, Yanzhong ^{[2
]}

Liu, Yuwei ^{[2
]}

Cheng, Yunhui ^{[1
]}

Chen, Xiuwen ^{[1
]}

Li, Wen ^{[1
]}

Wang, Shenming ^{[1
]}

Xiong, Ming ^{[2
]}

Kuzhikandathil, Eldo V. ^{[3
]}

Ye, Jiang-Hong ^{[2
]}

Zhang, Chunxiang ^{[1
]}

机构：

[1] Rush Univ, Med Ctr, Dept Pharmacol, Chicago, IL 60612 USA

[2] Univ Med & Dent New Jersey, New Jersey Med Sch, Dept Anesthesiol, Newark, NJ 07103 USA

[3] Univ Med & Dent New Jersey, New Jersey Med Sch, Dept Physiol & Pharmacol, Newark, NJ 07103 USA

来源：

EMBO MOLECULAR MEDICINE | 2013年 / 5卷 / 09期

关键词：

alcohol addiction; DeltaFosB; dopamine receptor D1; microRNAs; miR-382; VENTRAL TEGMENTAL AREA; CONDITIONED PLACE PREFERENCE; NEOINTIMAL LESION FORMATION; RAT NUCLEUS-ACCUMBENS; DELTA-FOSB INDUCTION; DOPAMINE D1; FOSB/DELTA-FOSB; RECEPTORS; BRAIN; STRIATUM;

D O I：

10.1002/emmm.201201900

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Alcohol addiction is a major social and health concern. Here, we determined the expression profile of microRNAs (miRNAs) in the nucleus accumbens (NAc) of rats treated with alcohol. The results suggest that multiple miRNAs were aberrantly expressed in rat NAc after alcohol injection. Among them, miR-382 was down-regulated in alcohol-treated rats. In both cultured neuronal cells in vitro and in the NAc in vivo, we identified that the dopamine receptor D1 (Drd1) is a direct target gene of miR-382. Via this target gene, miR-382 strongly modulated the expression of DeltaFosB. Moreover, overexpression of miR-382 significantly attenuated alcohol-induced up-regulation of DRD1 and DeltaFosB, decreased voluntary intake of and preference for alcohol and inhibited the DRD1-induced action potential responses. The results indicated that miRNAs are involved in and may represent novel therapeutic targets for alcoholism.

引用

页码：1402 / 1414

页数：13

共 46 条

[1] Nucleus accumbens dopamine D1 receptors regulate the expression of ethanol-induced behavioural sensitization [J].

Abrahao, Karina Possa ;

Quadros, Isabel Marian Hartmann ;

Oliveira Souza-Formigoni, Maria Lucia .

INTERNATIONAL JOURNAL OF NEUROPSYCHOPHARMACOLOGY, 2011, 14 (02) :175-185

[2] The functions of animal microRNAs [J].

Ambros, V .

NATURE, 2004, 431 (7006) :350-355

[3] Artificial microRNA-based neurokinin-1 receptor gene silencing reduces alcohol consumption in mice [J].

Baek, Mi Na ;

Jung, Kyoung Hwa ;

Halder, Debasish ;

Choi, Mi Ran ;

Lee, Byung-Hwa ;

Lee, Boung-Chul ;

Jung, Myung Hun ;

Choi, Ihn-Geun ;

Chung, Mi-Kyung ;

Oh, Dong-Yul ;

Chai, Young Gyu .

NEUROSCIENCE LETTERS, 2010, 475 (03) :124-128

[4] Involvement of nucleus accumbens dopamine D1 receptors in ethanol drinking, ethanol-induced conditioned place preference, and ethanol-induced psychomotor sensitization in mice [J].

Bahi, Amine ;

Dreyer, Jean-Luc .

PSYCHOPHARMACOLOGY, 2012, 222 (01) :141-153

[5] GENETIC VARIATION AND BRAIN GENE EXPRESSION IN RODENT MODELS OF ALCOHOLISM: IMPLICATIONS FOR MEDICATION DEVELOPMENT [J].

Bjork, Karl ;

Hansson, Anita C. ;

Sommer, Wolfgang H. .

FUNCTIONAL PLASTICITY AND GENETIC VARIATION: INSIGHTS INTO THE NEUROBIOLOGY OF ALCOHOLISM, 2010, 91 :129-171

[6] Differences in dopamine responsiveness to drugs of abuse in the nucleus accurnbens shell and core of Lewis and Fischer 344 rats [J].

Cadoni, Cristina ;

Di Chiara, Gaetano .

JOURNAL OF NEUROCHEMISTRY, 2007, 103 (02) :487-499

[7] Ethanol-induced sensitization depends preferentially on D1 rather than D2 dopamine receptors [J].

Camarini, Rosana ;

Marcourakis, Tania ;

Teodorov, Elizabeth ;

Yonamine, Mauricio ;

Calil, Helena Maria .

PHARMACOLOGY BIOCHEMISTRY AND BEHAVIOR, 2011, 98 (02) :173-180

[8] The evolution of gene regulation by transcription factors and microRNAs [J].

Chen, Kevin ;

Rajewsky, Nikolaus .

NATURE REVIEWS GENETICS, 2007, 8 (02) :93-103

[9] MicroRNAs are aberrantly expressed in hypertrophic heart - Do they play a role in cardiac hypertrophy? [J].

Cheng, Yunhui ;

Ji, Ruirui ;

Yue, Junming ;

Yang, Jian ;

Liu, Xiaojun ;

Chen, He ;

Dean, David B. ;

Zhang, Chunxiang .

AMERICAN JOURNAL OF PATHOLOGY, 2007, 170 (06) :1831-1840

[10] MicroRNA-145, a Novel Smooth Muscle Cell Phenotypic Marker and Modulator, Controls Vascular Neointimal Lesion Formation [J].

Cheng, Yunhui ;

Liu, Xiaojun ;

Yang, Jian ;

Lin, Ying ;

Xu, Da-Zhong ;

Lu, Qi ;

Deitch, Edwin A. ;

Huo, Yuqing ;

Delphin, Ellise S. ;

Zhang, Chunxiang .

CIRCULATION RESEARCH, 2009, 105 (02) :158-U113

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