A small-angle X-ray scattering study of the structure of lysozyme-sodium dodecyl sulfate complexes

被引：25

作者：

Narayanan, Janaky ^{[1
]}

Rasheed, A. S. Abdul ^{[1
]}

Bellare, Jayesh R. ^{[1
]}

机构：

[1] Indian Inst Technol, Dept Chem Engn, Bombay 400076, Maharashtra, India

来源：

JOURNAL OF COLLOID AND INTERFACE SCIENCE | 2008年 / 328卷 / 01期

关键词：

Protein-surfactant complex; Lysozyme; Sodium dodecyl sulfate; Micelles; Small-angle X-ray scattering;

D O I：

10.1016/j.jcis.2008.09.012

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

The Structure of lysozyme-sodium dodecyl sulfate (SIDS) complexes in Solution is Studied using small-angle X-ray scattering (SAXS). The SAXS data cannot be explained by the necklace and bead model for unfolded polypeptide chain interspersed with surfactant micelles. For the protein and surfactant concentrations used in the study, there is only marginal growth of SDS micelles as they complex with the protein. Being a small and rather rigid protein, lysozyme can penetrate the micellar core which is occupied by flexible and disordered paraffin chains and also the shell occupied by the hydrated head groups. A partially embedded swollen micellar model seems appropriate and describes well the scattering data. The SAXS intensity profiles are analyzed by considering the change in the electron scattering length density of the micellar core and shell due to complexation with protein and treating the intermicellar interaction using rescaled mean spherical approximation (RMSA) for charged spheres. (C) 2008 Elsevier Inc. All rights reserved

引用

页码：67 / 72

页数：6

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