Molecular cloning, expression and immunological characterisation of Pas n 1, the major allergen of Bahia grass Paspalum notatum pollen

被引:21
作者
Davies, Janet M. [1 ,2 ,3 ]
Mittag, Diana [1 ,2 ,3 ]
Dang, Thanh D. [1 ,2 ,3 ]
Symons, Karen [1 ,2 ]
Voskamp, Astrid [2 ,3 ]
Rolland, Jennifer M. [1 ,3 ]
O'Hehir, Robyn E. [1 ,2 ,3 ]
机构
[1] Univ Sydney, CRC Asthma & Airways, Sydney, NSW 2006, Australia
[2] Alfred Hosp, Dept Allergy Immunol & Resp Med, Melbourne, Vic 3004, Australia
[3] Monash Univ, Dept Immunol, AMREP, Alfred Hosp, Melbourne, Vic 3004, Australia
基金
英国医学研究理事会;
关键词
Group; 1; allergen; Pas n 1; Allergic rhinitis; Bahia grass pollen; Paspalum notatum;
D O I
10.1016/j.molimm.2008.08.267
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Bahia grass. Paspalum notatum, is a clinically important subtropical grass with a prolonged pollination season from spring to autumn. we aimed to clone and characterise the major Bahia grass pollen allergen, Pas n 1. Grass pollen-allergic patients presenting to a tertiary hospital allergy clinic were tested for IgE reactivity with Bahia grass pollen extract by skin prick testing, ImmunoCAP, ELISA and immunoblotting. Using primers deduced from the N-terminal peptide sequence ofa group 1 allergen of Bahia grass pollen extract separated by two-dimensional gel electrophoresis, the complete Pas n 1 cDNA was obtained by rapid amplification of cDNA ends and cloned. Biological relevance of recombinant Pas n 1 expressed in Escherichia coli was assessed by serum IgE reactivity and basophil activation. Twenty-nine of 34 (85%) consecutive patients presenting with grass pollen allergy were skin prick test positive to Bahia grass pollen. The Pas n 1 cDNA has sequence homology with the beta-expansin 1 glycoprotem family and is more closely related to the maize pollen group 1 allergen (85% identity) than to ryegrass Lol p 1 or Timothy grass Phl p 1 (64 and 66% identity, respectively). rPas n 1 reacted with serum IgE in 47 of 55 (85%) Bahia grass pollen-allergic patients, activated basophils and inhibited serum IgE reactivity with the 29 kDa band of Bahia grass pollen extract. In conclusion the cDNA for the major group 1 allergen of the subtropical Bahia grass pollen, Pas n 1, was identified and cloned. rPas n 1 is immunologically active and is a valuable reagent for diagnosis and specific immunotherapy of grass pollen allergy. (c) 2008 Elsevier Ltd. All rights reserved.
引用
收藏
页码:286 / 293
页数:8
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