The Role of TIR-NBS and TIR-X Proteins in Plant Basal Defense Responses

被引：128
作者：
Nandety, Raja Sekhar [1 ,2 ]
Caplan, Jeffery L. [2 ]
Cavanaugh, Keri [3 ]
Perroud, Bertrand [3 ]
Wroblewski, Tadeusz [3 ]
Michelmore, Richard W. [3 ]
Meyers, Blake C. [1 ,2 ]
机构：
[1] Univ Delaware, Dept Plant & Soil Sci, Newark, DE 19711 USA
[2] Univ Delaware, Delaware Biotechnol Inst, Newark, DE 19711 USA
[3] Univ Calif Davis, UC Davis Genome Ctr, Davis, CA 95616 USA
来源：
PLANT PHYSIOLOGY | 2013年 / 162卷 / 03期
基金：
美国国家卫生研究院; 美国国家科学基金会;
关键词：
SYSTEMIC ACQUIRED-RESISTANCE; DISEASE RESISTANCE; FUNCTIONAL-ANALYSIS; INNATE IMMUNITY; CELL-DEATH; ARABIDOPSIS; GENE; DOMAIN; ACTIVATION; TOBACCO;
D O I：
10.1104/pp.113.219162
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Toll/interleukin receptor (TIR) domain-containing proteins encoded in the Arabidopsis (Arabidopsis thaliana) genome include the TIR-nucleotide binding site (TN) and TIR-unknown site/domain (TX) families. We investigated the function of these proteins. Transient overexpression of five TX and TN genes in tobacco (Nicotiana benthamiana) induced chlorosis. This induced chlorosis was dependent on ENHANCED DISEASE RESISTANCE1, a dependency conserved in both tobacco and Arabidopsis. Stable overexpression transgenic lines of TX and TN genes in Arabidopsis produced a variety of phenotypes associated with basal innate immune responses; these were correlated with elevated levels of salicylic acid. The TN protein AtTN10 interacted with the chloroplastic protein phosphoglycerate dehydrogenase in a yeast (Saccharomyces cerevisiae) two-hybrid screen; other TX and TN proteins interacted with nucleotide binding-leucine-rich repeat proteins and effector proteins, suggesting that TN proteins might act in guard complexes monitoring pathogen effectors.
引用
收藏
页码：1459 / 1472
页数：14
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